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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Purified Concanavalin A acceptor glycoprotein from P815 cell line|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation. MA1-82739 does detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Intravenous infusion of monocytes isolated from 2-week-old mice enhances clearance of Beta-amyloid plaques in an Alzheimer mouse model.
MA1-82739 was used in flow cytometry to assess the effect of CD11b-positive (+) monocytes on Alzheimer's disease using a mouse model
|Hohsfield LA,Humpel C||PloS one (10:null)||2015|
CD68 antigen; LAMP4; macrophage antigen CD68; macrosialin; SCARD1; scavenger receptor class D member 1
Cd68; gp110; Lamp4; Scard1