|Flow Cytometry (Flow)||0.5 µg/test|
|Flow Cytometry (Flow)||See 20 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Mouse Human|
|Host / Isotype||Armenian hamster / IgG|
|Recommended Isotype Control||Armenian Hamster IgG Isotype Control, FITC, eBioscience™|
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.
Applications Reported: The H1.2F3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Also called AIM or activation inducer molecule, CD69 is a gp28/34 disulfide bonded homodimer with a Mr of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride. The CD69 molecule is also present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen. It is involved in lymphocyte signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD69; Early activation antigen CD69; Very Early Activation Antigen
Gene Aliases: 5830438K24Rik; AI452015; AIM; Cd69; VEA
UniProt ID: (Mouse) P37217
Entrez Gene ID: (Mouse) 12515
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