|Flow Cytometry (Flow)||1 µg/test|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2b, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: The FR70 monoclonal antibody reacts with mouse CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Applications Reported: This FR70 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This FR70 antibody has been tested by flow cytometric analysis of LPS-stimulated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD27 ligand; CD27-L; CD70; CD70 antigen; Ki-24 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7
Gene Aliases: Cd27l; CD27LG; Cd70; Tnfsf7
UniProt ID: (Mouse) O55237
Entrez Gene ID: (Mouse) 21948
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