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Invitrogen
CD71 (Transferrin Receptor) Monoclonal Antibody (MRQ-48), eBioscience™
FIGURE: 1 / 10
CD71 (Transferrin Receptor) Antibody (14-0718-93) in ICC/IF
Immunofluorescence analysis of CD71 was performed using 70% confluent log phase THP-1 cells differentiated into Macrophage (M0). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD71(Transferrin Receptor) Mouse Monoclonal Antibody (MRQ-48) (Product # 14-0718-... View More
-IHC-P-20170216130641.jpg?time=20220826)
-IHC-P-20170216130641.jpg?time=20220826?time=20220826 "CD71 (Transferrin Receptor) Antibody (14-0718-93) in IHC (P)")
Product Details
14-0718-93
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MRQ-48
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
tris, pH 7.3-7.7, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2637425
Product Specific Information
Description: The MRQ-48 monoclonal antibody recognizes human CD71, also known as the transferrin receptor. CD71 exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed on placental syncytiotrophoblasts, endocrine pancreas, hepatocytes, myocytes, basal keratinocytes, spermatocytes, and erythroid precursors. CD71 also is expressed by immature proliferating cells, at low levels on resting mature lymphocytes, and upregulated after antigen or mitogen stimulation of T and B cells. CD71 expression is used to aid in the identification of erythroid leukemia, benign erythroid proliferative disorders, and myelodysplastic syndrome.
Applications Reported: This MRQ-48 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunohistochemical staining.
Applications Tested: This MRQ-48 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval. This antibody can be used at less than or equal to 1:100. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD antigen CD71; CD71; p90; T9; TR; Transferrin receptor protein 1
Gene Aliases: CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR
UniProt ID: (Human) P02786
Entrez Gene ID: (Human) 7037
virus receptor activity
glycoprotein binding
double-stranded RNA binding
transferrin receptor activity
protein binding
Hsp70 protein binding
transferrin transmembrane transporter activity
identical protein binding
protein homodimerization activity
poly(A) RNA binding
chaperone binding
metalloprotease
regulation of cell growth
response to hypoxia
cellular iron ion homeostasis
acute-phase response
aging
response to nutrient
response to iron ion
response to manganese ion
osteoclast differentiation
positive regulation of B cell proliferation
receptor internalization
response to retinoic acid
transferrin transport
cellular response to drug
positive regulation of T cell proliferation
regulation of cell proliferation
positive regulation of bone resorption
positive regulation of isotype switching
response to copper ion
viral entry into host cell
iron ion import
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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