Invitrogen

CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), eFluor™ 450, eBioscience™

Product Image Gallery

CD73 Antibody in Flow Cytometry (Flow) — Staining of BALB/c splenocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) and 0.06 µg of Rat IgG1 K Isotype Control eFluor® 450 (Product # 48-4301-82) (left) or 0.06 µg of Anti-Mouse CD73 eFluor® 450 (right). Total viable cells were used for analysis.

CD73 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Immunocytochemistry and Immunohistochemistry

CD73 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow) — Fig. 1 Deficient CD73 and CD39 expressions and extracellular adenosine concentration in BM of OVX animals. ( A to K ) Characterization of healthy (sham) and OVX animals 4 weeks after ovariectomy. (A) Immunofluorescence staining of CD73 (green) and (B) CD39 (red) in vertebrae of OVX animals. Nuclear staining (blue). Scale bars, 100 mum. Inset shows magnified image of bone surface. Yellow arrowheads indicate cells positive for CD73 or CD39 on bone surface. Scale bars, 50 mum. (C) Flow cytometric analysis of CD73 and CD39 membrane expression of hematopoietic cells from mouse BM cells 4 weeks after ovariectomy (OVX) and healthy controls. (D) Percentage and median fluorescence intensity of hematopoietic cells expressing CD73. (E) Percentage and median fluorescence intensity of hematopoietic cells expressing CD39. (F) Flow cytometric analysis of CD73 and CD39 membrane expression of nonhematopoietic cells from mouse BM cells 4 weeks after ovariectomy and healthy controls. (G) Percentage and median fluorescence intensity of nonhematopoietic cells expressing CD73. (H) Percentage and median fluorescence intensity of nonhematopoietic cells expressing CD39. (I) CD73 gene expression and (J) CD39 gene expression of cells from bone chips. (K) Extracellular adenosine concentration in BM plasma of sham and OVX animals. n = 5. * P < 0.05, ** P < 0.01, *** P < 0.001.

CD73 Antibody in Immunohistochemistry (IHC) — Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Immunohistochemistry

Product Details

48-0731-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	0.06 µg/test	View 15 publications 15 publications

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG1
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 450, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBioTY/11.8 (TY/11.8)
Conjugate	eFluor™ 450 Functional Grade NovaFluor Red 685 NovaFluor Red 725 NovaFluor Red 755 NovaFluor Yellow 610 NovaFluor Yellow 690 NovaFluor Yellow 730 NovaFluor Yellow 755 PE PE-Cyanine7 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 702 Unconjugated
Excitation/Emission Max	405/445 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_1272196

Product Specific Information

Description: eBioTY/11.8 recognizes the with CD73 a 69-kDa GPI-anchored cell-surface protein with ecto-5'-nucleotidase activity. Expression on myeloid cells (CD11b) is restricted to the bone marrow. In human CD73 can be induced to secrete a soluble form with IL-2 suggesting a role in mediating activation signals. Differences between human and mouse CD73 have been reported. BALB/c mice have more CD4+CD73+ than CD8+CD73+ while the reciprocal is documented in humans.

CD73 is expressed on a subset of lymphocytes and increases during lymphocyte maturation. Recently it has been found that memory CD4 T cells express and are similar to the uncommitted primed precursor helper cells (Thpp) that can differentiate into Th1 or Th2 cells. Furthermore CD73 has been found on regulatory T cells.

Applications Reported: This eBioTY/11.8 (TY/11.8) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioTY/11.8 (TY/11.8) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD73 (Ecto-5-prime-nucleotidase, 5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. CD73 consists of a dimer of 2 identical 70 kDa subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. CD73 is used as a marker of lymphocyte differentiation. Consequently, a deficiency of CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from CD73 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations. Defects in the CD73 gene can lead to the calcification of joints and arteries, and intestinal tuberculosis. Two transcript variants encoding different isoforms of CD73 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

BMC musculoskeletal disorders

Xu W,Ni C,Wang Y,Zheng G,Zhang J,Xu Y

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Tue Apr 27 00:00:00 EDT 2021

Transplantation of bone marrow-derived mesenchymal stem cells with silencing of microRNA-138 relieves pelvic organ prolapse through the FBLN5/IL-1β/elastin pathway.

Aging

Zhao B,Sun Q,Fan Y,Hu X,Li L,Wang J,Cui S

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Sat Jan 16 00:00:00 EST 2021

Sema3A and HIF1α co-overexpressed iPSC-MSCs/HA scaffold facilitates the repair of calvarial defect in a mouse model.

Journal of cellular physiology

Li J,Wang T,Li C,Wang Z,Wang P,Zheng L

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Negative feedback control of neuronal activity by microglia.

Nature

Badimon A,Strasburger HJ,Ayata P,Chen X,Nair A,Ikegami A,Hwang P,Chan AT,Graves SM,Uweru JO,Ledderose C,Kutlu MG,Wheeler MA,Kahan A,Ishikawa M,Wang YC,Loh YE,Jiang JX,Surmeier DJ,Robson SC,Junger WG,Sebra R,Calipari ES,Kenny PJ,Eyo UB,Colonna M,Quintana FJ,Wake H,Gradinaru V,Schaefer A

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone loss.

Science advances

Shih YV,Liu M,Kwon SK,Iida M,Gong Y,Sangaj N,Varghese S

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Immunofluorescence

Thu Aug 01 00:00:00 EDT 2019

Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone loss.

Science advances

Shih YV,Liu M,Kwon SK,Iida M,Gong Y,Sangaj N,Varghese S

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Immunofluorescence

Thu Aug 01 00:00:00 EDT 2019

CD73 Promotes Glioblastoma Pathogenesis and Enhances Its Chemoresistance via A_2B Adenosine Receptor Signaling.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Yan A,Joachims ML,Thompson LF,Miller AD,Canoll PD,Bynoe MS

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Wed May 29 00:00:00 EDT 2019

VEGF expands erythropoiesis via hypoxia-independent induction of erythropoietin in noncanonical perivascular stromal cells.

The Journal of experimental medicine

Greenwald AC,Licht T,Kumar S,Oladipupo SS,Iyer S,Grunewald M,Keshet E

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Immunohistochemistry

Mon Jan 07 00:00:00 EST 2019

VEGF expands erythropoiesis via hypoxia-independent induction of erythropoietin in noncanonical perivascular stromal cells.

The Journal of experimental medicine

Greenwald AC,Licht T,Kumar S,Oladipupo SS,Iyer S,Grunewald M,Keshet E

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Immunohistochemistry

Mon Jan 07 00:00:00 EST 2019

PDGF is a potent initiator of bone formation in a tissue engineered model of pathological ossification.

Journal of tissue engineering and regenerative medicine

Davies OG,Grover LM,Lewis MP,Liu Y

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Calcium-mediated shaping of naive CD4 T-cell phenotype and function.

eLife

Guichard V,Bonilla N,Durand A,Audemard-Verger A,Guilbert T,Martin B,Lucas B,Auffray C

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Thu Dec 14 00:00:00 EST 2017

Three distinct developmental pathways for adaptive and two IFN-γ-producing γδ T subsets in adult thymus.

Nature communications

Buus TB,Ødum N,Geisler C,Lauritsen JPH

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Mon Dec 04 00:00:00 EST 2017

Accelerated craniofacial bone regeneration through dense collagen gel scaffolds seeded with dental pulp stem cells.

Scientific reports

Chamieh F,Collignon AM,Coyac BR,Lesieur J,Ribes S,Sadoine J,Llorens A,Nicoletti A,Letourneur D,Colombier ML,Nazhat SN,Bouchard P,Chaussain C,Rochefort GY

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Fri Dec 09 00:00:00 EST 2016

A Gain-Of-Function Mutation in the Plcg2 Gene Protects Mice from Helicobacter felis-Induced Gastric MALT Lymphoma.

PloS one

Gossmann J,Stolte M,Lohoff M,Yu P,Moll R,Finkernagel F,Garn H,Brendel C,Bittner A,Neubauer A,Huynh MQ

48073182 was used in flow cytometry to assess the impact of phospholipase C gamma 2 mutations to the development of gastric mucosa-associated lymphoid tissue lymphomas

Fri Nov 04 00:00:00 EDT 2016

Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.

Nature communications

Bemark M,Hazanov H,Strömberg A,Komban R,Holmqvist J,Köster S,Mattsson J,Sikora P,Mehr R,Lycke NY

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Tue Sep 06 00:00:00 EDT 2016

Novel role of cystic fibrosis transmembrane conductance regulator in maintaining adult mouse olfactory neuronal homeostasis.

The Journal of comparative neurology

Pfister S,Weber T,Härtig W,Schwerdel C,Elsaesser R,Knuesel I,Fritschy JM

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Immunofluorescence

Sun Feb 15 00:00:00 EST 2015

Cell-autonomous heparanase modulates self-renewal and migration in bone marrow-derived mesenchymal stem cells.

Journal of biomedical science

Cheng CC,Lee YH,Lin SP,Huangfu WC,Liu IH

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Thu Mar 13 00:00:00 EDT 2014

Multipotent stem cells from trabecular meshwork become phagocytic TM cells.

Investigative ophthalmology & visual science

Du Y,Roh DS,Mann MM,Funderburgh ML,Funderburgh JL,Schuman JS

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Wed Mar 21 00:00:00 EDT 2012

Adipose-derived stem cells promote lymphangiogenesis in response to VEGF-C stimulation or TGF-β1 inhibition.

Future oncology (London, England)

Yan A,Avraham T,Zampell JC,Haviv YS,Weitman E,Mehrara BJ

Published figure using CD73 monoclonal antibody (Product # 48-0731-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: 5'-NT; 5'-nucleotidase; CD73; Ecto-5'-nucleotidase

Gene Aliases: 2210401F01Rik; AI447961; CD73; eNT; NT; Nt5; Nt5e; Nte

UniProt ID: (Mouse) Q61503

Entrez Gene ID: (Mouse) 23959

Molecular Function: phosphodiesterase

Function(s)

purine nucleotide biosynthetic process AMP catabolic process leukocyte cell-cell adhesion nucleotide catabolic process dephosphorylation adenosine metabolic process adenosine biosynthetic process negative regulation of inflammatory response

Process(es)

nucleotide binding ferrous iron binding 5'-nucleotidase activity hydrolase activity hydrolase activity, acting on ester bonds metal ion binding

CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), eFluor™ 450, eBioscience™

Product Details

48-0731-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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