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Immunofluorescence analysis of CD79a / Neurofilament-H was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD79a (2F11) / Neurofilament-H Mouse Monoclonal Antibody (Product # MA5-15234) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Cat, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Avian, Rabbit, Fish|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Neurofilament purified from human brain|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15234 targets Neurofilament (200kDa and 68kDa) in IF, IHC (P), IP, and WB applications and shows reactivity with Feline, Human, mouse, Rabbit, and Rat samples.
The MA5-15234 immunogen is neurofilament purified from human brain.
Neurofilaments are the intermediate filaments of neurons and their processes. They are protein triplets composed of three major subunits of MW 68kDa, 155kDa, and 200kDa. Neurofilaments are expressed in tumors of neural origin, or displaying neuronal differentiation, such as neuroblastoma, medulloblastoma, and retinoblastoma
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protective effect of Antrodia camphorata on bladder ischemia/reperfusion injury.
MA5-15234 was used in immunohistochemistry to study the protective effect of Antrodia camphorata in bladder ischemia/reperfusion injury
|Juan YS,Mannikarottu A,Chuang SM,Li S,Lin AD,Chang-Chou L,Schuler C,Leggett RE,Levin RM||International urology and nephrology (42:637)||2010|
Coenzyme Q10 diminishes ischemia-reperfusion induced apoptosis and nerve injury in rabbit urinary bladder.
MA5-15234 was used in immunohistochemistry to study the role of Coenzyme Q in reducing ischemia-reperfusion-induced apoptosis and nerve damage in rabbit bladder
|Juan YS,Chuang SM,Mannikarottu A,Huang CH,Li S,Schuler C,Levin RM||Neurourology and urodynamics (28:339)||2009|
Free radical damage as a biomarker of bladder dysfunction after partial outlet obstruction and reversal.
MA5-15234 was used in immunohistochemistry to study protein carbonylation and nitrotyrosination as a biomarker of dysfunction after partial bladder outlet obstruction.
|Lin WY,Guven A,Juan YS,Neuman P,Whitbeck C,Chichester P,Kogan B,Levin RM,Mannikarottu A||BJU international (101:621)||2008|
Effects of L-arginine and L-NAME on chronic partial bladder outlet obstruction in rabbit.
MA5-15234 was used in immunohistochemistry to investigate the impact of nitric oxide synthase and blook flow on the severity of damages caused by acute and chronic partial bladder outlet obstruction
|Lin WY,Levin RM,Chichester P,Leggett R,Juan YS,Johnson A,Neumann P,Whitbeck C,Guven A,Kogan B,Mannikarottu A||American journal of physiology. Regulatory, integrative and comparative physiology (293:R2390)||2007|
Electrophysiological measurements in three-dimensional in vivo-mimetic organotypic cell cultures: preliminary studies with hen embryo brain spheroids.
MA5-15234 was used in immunohistochemistry to investigate the electrophysiological properties of three-dimensional hen embryo brain spheroids
|Uroukov IS,Ma M,Bull L,Purcell WM||Neuroscience letters (404:33)||2006|
L-NAME, a nitric oxide synthase inhibitor, diminishes oxidative damage in urinary bladder partial outlet obstruction.
MA5-15234 was used in immunohistochemistry to investigate the effect of the nitric oxide synthase inhibitor L-NAME on oxidative damage
|Conners W,Whitebeck C,Chicester P,Legget R,Lin AD,Johnson A,Kogan B,Levin R,Mannikarottu A||American journal of physiology. Renal physiology (290:F357)||2006|
Protection of urinary bladder function by grape suspension.
MA5-15234 was used in immunohistochemistry to investigate urinary bladder dysfunction in a rabbit model
|Agartan CA,Whitbeck C,Sokol R,Chichester P,Levin RM||Phytotherapy research : PTR (18:1013)||2004|
Persistent infection of betanodavirus in a novel cell line derived from the brain tissue of barramundi Lates calcarifer.
MA5-15234 was used in immunocytochemistry to establish and characterize a novel cell line infected with betanodavirus
|Chi SC,Wu YC,Cheng TM||Diseases of aquatic organisms (65:91)||2005|
B-cell antigen receptor complex-associated protein alpha chain; CD79A antigen (immunoglobulin-associated alpha); CD79a antigen immunoglobulin-associated alpha; CD79a molecule, immunoglobulin-associated alpha; Ig alpha; ig-alpha; IGA; immunoglobulin-associated alpha; MB-1; MB-1 membrane glycoprotein; membrane-bound immunoglobulin-associated protein; surface IgM-associated protein
CD79A; Ig-alpha; IGA; Igalpha; Ly-54; Ly54; MB-1; MB1