Immunofluorescence analysis of CD79a was performed using 70% confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD79a Mouse (HM47/A9) Monoclonal Antibody (Product # MA5-13212) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Bovine, Chicken, Horse, Guinea pig, Human, Mouse, Non-human primate, Pig, Rabbit, Rat|
|Published species reactivity||Dog, Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A synthetic peptide consisting of GTYQDVGSLNIADVQ of human CD79a protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1.0 µg/ml|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 6 publications below|
MA5-13212 targets CD79a in IF, IHC (P), and WB applications and shows reactivity with Bovine, Equine, Guinea Pig, Human, mouse, Non-human primate, Porcine, Rabbit, Chicken, and Rat samples.
The MA5-13212 immunogen is a synthetic peptide consisting of GTYQDVGSLNIADVQ of human CD79a protein.
MA5-13212 is not functional in IHC (P) in its B format.
A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Lymphocytic leukemia in a captive dhole (Cuon alpinus).
MA5-13212 was used in immunohistochemistry to report on a case of lymphocytic leukemia in a captive dhole
|Scala C,Ortiz K,Nicolier A,Briend-Marchal A||Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians (44:204)||2013|
Blastic plasmacytoid dendritic cell neoplasm with leukemic presentation: an Italian multicenter study.
MA5-13212 was used in immunohistochemistry to evaluate 43 cases of blastic plasmacytoid dendritic cell neoplasm with leukemic presentation
|Pagano L,Valentini CG,Pulsoni A,Fisogni S,Carluccio P,Mannelli F,Lunghi M,Pica G,Onida F,Cattaneo C,Piccaluga PP,Di Bona E,Todisco E,Musto P,Spadea A,D'Arco A,Pileri S,Leone G,Amadori S,Facchetti F||Haematologica (98:239)||2013|
Rats deficient for p53 are susceptible to spontaneous and carcinogen-induced tumorigenesis.
MA5-13212 was used in immunohistochemistry to study the susceptibility to spontaneous and carcinogen-induced tumorigenesis in p53-deficient rats
|Yan HX,Wu HP,Ashton C,Tong C,Ying QL||Carcinogenesis (33:2001)||2012|
Pneumocystis mediates overexpression of antizyme inhibitor resulting in increased polyamine levels and apoptosis in alveolar macrophages.
MA5-13212 was used in immunohistochemistry to study the mechanism of increased polyamine production and apoptosis during Pneumocystis pneumonia
|Liao CP,Lasbury ME,Wang SH,Zhang C,Durant PJ,Murakami Y,Matsufuji S,Lee CH||The Journal of biological chemistry (284:8174)||2009|
A tumor-suppressor function for NFATc3 in T-cell lymphomagenesis by murine leukemia virus.
MA5-13212 was used in immunohistochemistry to investigate the involvement NFATc3 in T-cell lymphomagenesis by murine leukemia virus
|Glud SZ,Sørensen AB,Andrulis M,Wang B,Kondo E,Jessen R,Krenacs L,Stelkovics E,Wabl M,Serfling E,Palmetshofer A,Pedersen FS||Blood (106:3546)||2005|
Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors.
MA5-13212 was used in immunohistochemistry to study histochemical and immunohistochemical stains for differentiating canine cutaneous round cell tumors
|Fernandez NJ,West KH,Jackson ML,Kidney BA||Veterinary pathology (42:437)||2005|