|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||IgM complex isolated from Daudi cells.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||10 ug/ml|
|Immunoprecipitation (IP)||20 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-81093 reacts with human CD79a-positive cells by flow cytometry and ELISA specific for a fusion protein of CD79a-Fc. This clone has been reported to be useful in distinguishing B-CLL from mantle cell lymphoma in flow cytometric assays.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines.
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