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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||human peripheral blood T lymphocytes|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
FACS procedure using PBMCs: Add 10µl of MA1-12030/10^6 PBMC in 100µl PBS. Mix gently and incubate for 15 minutes on ice. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
FACS procedure using whole blood: Add 10µl of MA1-12030/100µl of whole blood. Mix gently and incubate for 15 minutes at room temperature. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD8; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; Leu-2; Leu2 T-lymphocyte antigen; MAL; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen
CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37