|Tested species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat/mouse T cell hybrids expressing CD8.|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/1 million cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-70040 detects CD8b in rat samples.
MA1-70040 has been successfully used in flow cytometry procedures.
The MA1-70040 immunogen is rat/mouse T cell hybrids expressing CD8.
This antibody recognizes CD8. CD8 is composed of an alpha chain with Mr=30-34 kDa linked by disulfide bonds to a beta chain with Mr=30-32 kDa. In addition to forming heterodimers with the beta chain, the alpha chain is also capable of forming homodimers while the beta chain is incapable of forming homodimers. The extracellular portion of CD8alpha binds to the non-polymorphic portion of MHC class I molecules. This interaction is important in the antigen presentation to CD8+ T cells. The cytoplasmic domain of the alpha chain associates with p56lck, a member of the Src family of tyrosine kinases. Cells which are reactive with this antibody include: thymocytes, natural killer cells, and a subset (CD4-) of peripheral T-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.