Figure 1. Multicolor analysis of CD4-positive and CD8-positive populations using Qdot® 605 streptavidin and a Pacific Blue™ dye–labeled conjugate. Human mononuclear cells were blocked with nonspecific goat IgG and then stained with a biotinylated mouse anti–human CD4 antibody. Cells were incubated with Qdot® 605 streptavidin, then with mouse anti–human CD8 antibody conjugated to Pacific Blue™ dye. Cells were analyzed on a flow cytometer equipped with a 405 nm violet diode laser and 450/50 and 605/20 nm bandpass filters. Compensation was performed using single-color controls. Cells were analyzed using a lymphocyte gate as determined by FSC/SSC. These reagents give clear separation of the CD4-positive and CD8-positive populations using a single excitation source, the violet diode laser.
|Tested species reactivity||Human|
|Published species reactivity||Human, Rhesus monkey|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recognizes the CD8 antigen|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. CD38 (T10) is a single chain 46 kDa type II integral glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.
MHCD0828 was used in flow cytometry to develop and validate a novel system for high throughput quantitation of autophagy in primary human leukocytes.
|Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK||Autophagy (8:677)||2012|
|Rhesus monkey||Not Cited||
A CD8¿(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities.
MHCD0828 was used in flow cytometry to characterize different macaque NK cell subpopulations.
|Vargas-Inchaustegui DA,Demberg T,Robert-Guroff M||Immunology (134:326)||2011|