|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable, Rhesus monkey|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recognizes the CD8 antigen|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. CD38 (T10) is a single chain 46 kDa type II integral glycoprotein with a short N terminal cytoplasmic tail. CD38 is highly expressed on thymocytes. It is also expressed by early cells of B and T lineages, NK cells, plasma cells, monocytes and macrophages and may be detected on cells from multiple myeloma, ALL (B and T) and some AML. In normal lymph nodes and tonsils the antigen is detected mainly on B cells in germinal centers and in plasma cells. The extracellular domain of the molecule shares a high homology sequence with Aplysia ADP ribosyl cyclase. CD38 functions as a multicatalytic ectoenzyme serving as ADP ribosyl cyclase, ADPR hydrolase and possibly NAD+ glycohydrolase, or as a surface receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
¿¿ T cells and CD14+ monocytes are predominant cellular sources of cytokines and chemokines associated with severe malaria.
Q10009 was used in flow cytometry to investigate the cellular sources of cytokines and chemokines associated with severe malaria
|Stanisic DI,Cutts J,Eriksson E,Fowkes FJ,Rosanas-Urgell A,Siba P,Laman M,Davis TM,Manning L,Mueller I,Schofield L||The Journal of infectious diseases (210:295)||2014|
Subinfectious hepatitis C virus exposures suppress T cell responses against subsequent acute infection.
Q10009 was used in flow cytometry to decipher how susequent acute infection is supressed in T cell responses after exposure to subinfectious hepatitis C virus
|Park SH,Veerapu NS,Shin EC,Biancotto A,McCoy JP,Capone S,Folgori A,Rehermann B||Nature medicine (19:1638)||2013|
Increased expression of intrinsic antiviral genes in HLA-B*57-positive individuals.
Q10009 was used in flow cytometry to report the associations among host restriction factors, other established immunological parameters, and HLA type in HIV-1-seronegative individuals
|Raposo RA,Abdel-Mohsen M,Holditch SJ,Kuebler PJ,Cheng RG,Eriksson EM,Liao W,Pillai SK,Nixon DF||Journal of leukocyte biology (94:1051)||2013|
Expression profile of host restriction factors in HIV-1 elite controllers.
Q10009 was used in flow cytometry to investigate cellular restriction of HIV-1
|Abdel-Mohsen M,Raposo RA,Deng X,Li M,Liegler T,Sinclair E,Salama MS,Ghanem Hel-D,Hoh R,Wong JK,David M,Nixon DF,Deeks SG,Pillai SK||Retrovirology (10:null)||2013|
The contribution of non-conventional T cells and NK cells in the mycobacterial-specific IFN¿ response in Bacille Calmette-Guérin (BCG)-immunized infants.
Q10009 was used in flow cytometry to discuss the roles of TCR-gamma/delta T cells and natural killer cells in the mycobacterial-specific immune response
|Zufferey C,Germano S,Dutta B,Ritz N,Curtis N||PloS one (8:null)||2013|
|Rhesus monkey||Not Cited||
DNA-based HIV vaccines do not induce generalized activation in mucosal tissue T cells.
Q10009 was used in flow cytometry to test if vaccination alters the activation state of T cells within the gastrointestinal mucosa, inguinal lymph nodes, and peripheral blood
|Reuter MA,Yuan S,Marx PA,Kutzler MA,Weiner DB,Betts MR||Human vaccines and immunotherapeutics (8:1648)||2012|
|Not Applicable||Not Cited||
Leukocyte composition of human breast cancer.
Q10009 was used in flow cytometry to examine tumor and nonadjacent normal breast tissue from women with breast cancer, who either had or had not received neoadjuvant chemotherapy before surgery
|Ruffell B,Au A,Rugo HS,Esserman LJ,Hwang ES,Coussens LM||Proceedings of the National Academy of Sciences of the United States of America (109:2796)||2012|
Increased frequency of regulatory T cells accompanies increased immune activation in rectal mucosae of HIV-positive noncontrollers.
Q10009 was used in flow cytometry to study the role of T regulatory cells in HIV infection
|Shaw JM,Hunt PW,Critchfield JW,McConnell DH,Garcia JC,Pollard RB,Somsouk M,Deeks SG,Shacklett BL||Journal of virology (85:11422)||2011|
CD4+CD8+ T cells represent a significant portion of the anti-HIV T cell response to acute HIV infection.
Q10009 was used in flow cytometry to analyze the proliferation and functional profile of circulating double positive T cells from acutely HIV-infected individuals and chronically HIV-infected viral controllers.
|Frahm MA,Picking RA,Kuruc JD,McGee KS,Gay CL,Eron JJ,Hicks CB,Tomaras GD,Ferrari G||Journal of immunology (Baltimore, Md. : 1950) (188:4289)||2012|