|Tested species reactivity||Human|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:2|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13473 targets CD8 in IHC (P) and FACSs applications and shows reactivity with Human samples.
The MA5-13473 immunogen is a 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule.
CD8 molecule consists of two chains, termed alpha and beta chain, which are expressed as a disulphide-alpha beta heterdimer or as an alpha alpha homodimer on T cell subset (cytotoxic/suppressor T cells), thymocytes and NK cells. The majority of CD8+ Tcells expresses CD8 as alphabetaheterodimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 alpha chain (but not beta chain).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Analysis of Immune Signatures in Longitudinal Tumor Samples Yields Insight into Biomarkers of Response and Mechanisms of Resistance to Immune Checkpoint Blockade.
MA5-13473 was used in immunohistochemistry - paraffin section to gain insight into biomarkers of response and mechanisms of resistance to immune checkpoint blockade by analysis of immune signatures in longitudinal tumor samples
|Chen PL,Roh W,Reuben A,Cooper ZA,Spencer CN,Prieto PA,Miller JP,Bassett RL,Gopalakrishnan V,Wani K,De Macedo MP,Austin-Breneman JL,Jiang H,Chang Q,Reddy SM,Chen WS,Tetzlaff MT,Broaddus RJ,Davies MA,Gershenwald JE,Haydu L,Lazar AJ,Patel SP,Hwu P,Hwu WJ,Diab A,Glitza IC,Woodman SE,Vence LM,Wistuba II,Amaria RN,Kwong LN,Prieto V,Davis RE,Ma W,Overwijk WW,Sharpe AH,Hu J,Futreal PA,Blando J,Sharma P,Allison JP,Chin L,Wargo JA||Cancer discovery (6:827)||2016|
Analysis of FoxP3+ T-regulatory cells and CD8+ T-cells in ovarian carcinoma: location and tumor infiltration patterns are key prognostic markers.
MA5-13473 was used in immunohistochemistry - paraffin section to characterize location and tumor infiltration patterns as key prognostic factors when analyzing CD8+ T-cells and FoxP3+ T-regulatory cells in ovarian carcinoma
|Hermans C,Anz D,Engel J,Kirchner T,Endres S,Mayr D||PloS one (9:null)||2014|
Histopathological features of sinonasal inverted papillomas in chinese patients.
MA5-13473 was used in immunohistochemistry - paraffin section to examine the histopathological patterns and their associations with clinical characteristics in Chinese patients with nasal inverted papilloma
|Zhao L,Li CW,Jin P,Ng CL,Lin ZB,Li YY,Li TY,Petersson BF,Shi L,Wang de Y||The Laryngoscope (126:E141)||2016|
Smoking is an independent association of squamous metaplasia in Chinese nasal polyps.
MA5-13473 was used in immunohistochemistry - paraffin section to characterize Chinese nasal polyp
|Gao T,Ng CL,Li C,Li YY,Duan C,Shen L,Jiao YF,Liu M,Wang de Y||International forum of allergy and rhinology (6:66)||2016|
A viral regulator of glycoprotein complexes contributes to human cytomegalovirus cell tropism.
MA5-13473 was used in immunoprecipitation to investigate the assembly of glycoprotein complexes by human cytomegalovirus.
|Li G,Nguyen CC,Ryckman BJ,Britt WJ,Kamil JP||Proceedings of the National Academy of Sciences of the United States of America (112:4471)||2015|
The differential diagnosis of CD8-positive ("type D") lymphomatoid papulosis.
MA5-13473 was used in immunohistochemistry to study histopathological features that may aid in the differential diagnosis of CD8+ lymphomatoid papulosus
|McQuitty E,Curry JL,Tetzlaff MT,Prieto VG,Duvic M,Torres-Cabala C||Journal of cutaneous pathology (41:88)||2014|
Viral acute lower respiratory infections impair CD8+ T cells through PD-1.
MA5-13473 was used in immunohistochemistry to study the role of PD-1 in the mechanism by which viral acute lower respiratory tract infections impair CD8+ T-cell function
|Erickson JJ,Gilchuk P,Hastings AK,Tollefson SJ,Johnson M,Downing MB,Boyd KL,Johnson JE,Kim AS,Joyce S,Williams JV||The Journal of clinical investigation (122:2967)||2012|
Leukocyte composition of human breast cancer.
MA5-13473 was used in immunohistochemistry to examine tumor and nonadjacent normal breast tissue from women with breast cancer, who either had or had not received neoadjuvant chemotherapy before surgery
|Ruffell B,Au A,Rugo HS,Esserman LJ,Hwang ES,Coussens LM||Proceedings of the National Academy of Sciences of the United States of America (109:2796)||2012|
In situ B cell-mediated immune responses and tubulointerstitial inflammation in human lupus nephritis.
MA5-13473 was used in immunohistochemistry to study the involvement of B cell-mediated immune responses in the pathogenesis of lupus tubulointerstitial inflammation
|Chang A,Henderson SG,Brandt D,Liu N,Guttikonda R,Hsieh C,Kaverina N,Utset TO,Meehan SM,Quigg RJ,Meffre E,Clark MR||Journal of immunology (Baltimore, Md. : 1950) (186:1849)||2011|
Memory B-cell aggregates in skin biopsy are diagnostic for primary Sjögren's syndrome.
MA5-13473 was used in immunohistochemistry to investigate the use of B-cell skin infiltrates in the diagnosis of Sjogren's syndrome.
|Roguedas AM,Pers JO,Lemasson G,Devauchelle V,Tobón GJ,Saraux A,Misery L,Youinou P||Journal of autoimmunity (35:241)||2010|
Tracking the rejection and survival of mouse ovarian iso- and allografts in vivo with bioluminescent imaging.
MA5-13473 was used in immunohistochemistry to study the use of bioluminescent imaging in transgenic mice to monitor the survival and rejection of ovarian isografts and allografts
|Chen CH,Yeh YC,Wu GJ,Huang YH,Lai WF,Liu JY,Tzeng CR||Reproduction (Cambridge, England) (140:105)||2010|
Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy--a case report.
MA5-13473 was used in immunohistochemistry to report on a colon cancer patient treated with repeated infusions of ex vivo TKD/IL-2-activated NK cells
|Milani V,Stangl S,Issels R,Gehrmann M,Wagner B,Hube K,Mayr D,Hiddemann W,Molls M,Multhoff G||Journal of translational medicine (7:null)||2009|
Local immune alterations in oral mucosa of the edentulous patients; possible cause of teeth loss?
MA5-13473 was used in immunohistochemistry to investigate immune cells of oral mucosa derived from edentulous patients
|Zurac S,Girtan M,Lavric L,Petsakos G,St¿niceanu F,Bastian A,Andrei R,Popp C,Laba E||Romanian journal of internal medicine = Revue roumaine de medecine interne (46:249)||2009|
BAFF-modulated repopulation of B lymphocytes in the blood and salivary glands of rituximab-treated patients with Sjögren's syndrome.
MA5-13473 was used in immunohistochemistry to investigate the mechanism for the repopulation of B lymphocytes after rituximab treatment
|Pers JO,Devauchelle V,Daridon C,Bendaoud B,Le Berre R,Bordron A,Hutin P,Renaudineau Y,Dueymes M,Loisel S,Berthou C,Saraux A,Youinou P||Arthritis and rheumatism (56:1464)||2007|
CD8 tumor-infiltrating lymphocytes are predictive of survival in muscle-invasive urothelial carcinoma.
MA5-13473 was used in immunohistochemistry to study the prognostic value of CD8 tumor-infiltrating lymphocytes in muscle-invasive urothelial carcinoma
|Sharma P,Shen Y,Wen S,Yamada S,Jungbluth AA,Gnjatic S,Bajorin DF,Reuter VE,Herr H,Old LJ,Sato E||Proceedings of the National Academy of Sciences of the United States of America (104:3967)||2007|
Intraepithelial CD8+ tumor-infiltrating lymphocytes and a high CD8+/regulatory T cell ratio are associated with favorable prognosis in ovarian cancer.
MA5-13473 was used in immunohistochemistry to study the prognostic value of intraepithelial CD8+ tumor-infiltrating lymphocytes and a high CD8+/regulatory T cell ratio in ovarian cancer
|Sato E,Olson SH,Ahn J,Bundy B,Nishikawa H,Qian F,Jungbluth AA,Frosina D,Gnjatic S,Ambrosone C,Kepner J,Odunsi T,Ritter G,Lele S,Chen YT,Ohtani H,Old LJ,Odunsi K||Proceedings of the National Academy of Sciences of the United States of America (102:18538)||2005|
Immunohistochemical analysis of orbital connective tissue specimens of patients with active Graves ophthalmopathy.
MA5-13473 was used in immunohistochemistry to study the cells infiltrating orbital connective tissue in patients with active Graves opthalmopathy
|Avunduk AM,Avunduk MC,Pazarli H,Oguz V,Varnell ED,Kaufman HE,Aksoy F||Current eye research (30:631)||2005|
Etanercept treatment in the endotoxin-induced uveitis of rats.
MA5-13473 was used in immunohistochemistry to investigate the therapeutic effect of etanercept on endotoxin-induced uveitis in rats
|Avunduk MC,Avunduk AM,Oztekin E,Baltaci AK,Ozyazgan Y,Mogolkoc R||Experimental eye research (79:357)||2004|