|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Normal human peripheral blood lymphocytes.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:100|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
NK cells as effectors of acquired immune responses: effector CD4+ T cell-dependent activation of NK cells following vaccination.
MA1-81127 was used in flow cytometry to study the molecular mechanisms that regulate the "recall" NK cell response after rabies vaccination
|Horowitz A,Behrens RH,Okell L,Fooks AR,Riley EM||Journal of immunology (Baltimore, Md. : 1950) (185:2808)||2010|