|Tested species reactivity||Rat|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||High molecular weight rat thymocyte.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/1 million cells|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-70003 detects CD8a in rat samples.
MA1-70003 has been successfully used in flow cytometry, and immunohistochemistry procedures.
The MA1-70003 immunogen is high molecular weight rat thymocyte.
This antibody recognizes CD8. CD8 is composed of an alpha chain with Mr=30-34 kDa linked by disulfide bonds to a beta chain with Mr=30-32 kDa. In addition to forming heterodimers with the beta chain, the alpha chain is also capable of forming homodimers while the beta chain is incapable of forming homodimers. The extracellular portion of CD8alpha binds to the non-polymorphic portion of MHC class I molecules. This interaction is important in the antigen presentation to CD8+ T cells. The cytoplasmic domain of the alpha chain associates with p56lck, a member of the Src family of tyrosine kinases. Cells which are reactive with this antibody include: thymocytes, natural killer cells, and a subset (CD4-) of peripheral T-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Eradication of rat malignant gliomas by retroviral-mediated, in vivo delivery of the interleukin 4 gene.
MA1-70003 was used in immunohistochemistry to test if IL-4 can protect from malignant gliomas in vivo.
|Benedetti S,Bruzzone MG,Pollo B,DiMeco F,Magrassi L,Pirola B,Cirenei N,Colombo MP,Finocchiaro G||Cancer research (59:645)||1999|
Limited efficacy of the HSV-TK/GCV system for gene therapy of malignant gliomas and perspectives for the combined transduction of the interleukin-4 gene.
MA1-70003 was used in immunohistochemistry to study the effects of IL-4 using the HSV-tk/GCV system of glioma gene therapy.
|Benedetti S,Dimeco F,Pollo B,Cirenei N,Colombo BM,Bruzzone MG,Cattaneo E,Vescovi A,Didonato S,Colombo MP,Finocchiaro G||Human gene therapy (8:1345)||1997|
Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations.
MA1-70003 was used in flow cytometry to investigate the expression of prolactin receptor in lymphocyte subsets
|Gagnerault MC,Touraine P,Savino W,Kelly PA,Dardenne M||Journal of immunology (Baltimore, Md. : 1950) (150:5673)||1993|
|Not Applicable||0.5 mg/ml||
Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens.
MA1-70003 was used in immunoprecipitation to use W3/25 and MRC OX-8 to study rat T cell populations
|Thomas ML,Green JR||European journal of immunology (13:855)||1983|