Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of the cytoplasmic domain of alpha chain of the CD8 molecule|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.
CD8 molecule consists of two chains, termed a and ß chain, which are expressed as a disulphidelinked a/ß heterdimer or as an a/a homodimer on T cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as a/ß heterdimer. CD8 functions as a coreceptor in concertrt with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 a chain (but not ß chain).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD8; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; Leu-2; Leu2 T-lymphocyte antigen; MAL; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen
CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37