|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide sequence comprising the 13 C-terminal amino acids of the cytoplasmic domain of alpha chain of the CD8 molecule|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500-1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 3 publications below|
PA5-16693 targets CD8 in IHC (P) applications and shows reactivity with Human samples.
The PA5-16693 immunogen is synthetic peptide sequence comprising the 13 C-terminal amino acids of the cytoplasmic domain of alpha chain of the CD8 molecule.
CD8 molecule consists of two chains, termed alpha and Beta chain, which are expressed as a disulphide-linked alpha/Beta heterdimer or as an alpha/alpha homodimer on T cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as alpha/Beta heterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 alpha chain (but not Beta chain).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The ratio of CD8 to Treg tumor-infiltrating lymphocytes is associated with response to cisplatin-based neoadjuvant chemotherapy in patients with muscle invasive urothelial carcinoma of the bladder.
PA5-16693 was used in immunohistochemistry to study the prognostic value of the ratio of CD8 to Treg tumor-infiltrating lymphocyte in bladder cancer
|Baras AS,Drake C,Liu JJ,Gandhi N,Kates M,Hoque MO,Meeker A,Hahn N,Taube JM,Schoenberg MP,Netto G,Bivalacqua TJ||Oncoimmunology (5:null)||2016|
MHC-I expression renders catecholaminergic neurons susceptible to T-cell-mediated degeneration.
PA5-16693 was used in immunohistochemistry to study T-cell mediated degeneration of catacholaminergic neurons expressing MHC I
|Cebrián C,Zucca FA,Mauri P,Steinbeck JA,Studer L,Scherzer CR,Kanter E,Budhu S,Mandelbaum J,Vonsattel JP,Zecca L,Loike JD,Sulzer D||Nature communications (5:null)||2014|
Immune response to Mycobacterium tuberculosis infection in the parietal pleura of patients with tuberculous pleurisy.
PA5-16693 was used in immunohistochemistry to report the immune responses in the parietal pleura of patients with tuberculous pleurisy and compare them with nonspecific pleuritis
|Caramori G,Lasagna L,Casalini AG,Adcock IM,Casolari P,Contoli M,Tafuro F,Padovani A,Chung KF,Barnes PJ,Papi A,Rindi G,Bertorelli G||PloS one (6:null)||2011|