|Tested species reactivity||Human|
|Published species reactivity||Rat, Human, Not Applicable|
|Host / Isotype||Rabbit|
|Immunogen||Synthetic peptide sequence comprising the 13 C-terminal amino acids of the cytoplasmic domain of alpha chain of the CD8 molecule|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:2|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:1-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14548 targets CD8 in IHC (P), FACS and WB applications and shows reactivity with Human samples. This antibody does not react with mouse tissue in Western blot applications.
The MA5-14548 immunogen is synthetic peptide sequence comprising the 13 C-terminal amino acids of the cytoplasmic domain of alpha chain of the CD8 molecule.
CD8 molecule consists of two chains, termed alpha and beta chain, which are expressed as a disulphide-linked alpha/beta heterdimer or as an alpha/alpha homodimer on T cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as alpha/beta heterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 alpha chain (but not beta chain).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Evidence of a pathogenic role for CD8(+) T cells in anti-GABAB receptor limbic encephalitis.
MA5-14548 was used in immunohistochemistry to characterize anti-GABAB receptor limbic encephalitis and evidence of a pathogenic role for CD8(+) T cells
|Golombeck KS,Bönte K,Mönig C,van Loo KM,Hartwig M,Schwindt W,Widman G,Lindenau M,Becker AJ,Glatzel M,Elger CE,Wiendl H,Meuth SG,Lohmann H,Gross CC,Melzer N||Neurology(R) neuroimmunology and neuroinflammation (3:null)||2016|
Low intraprostatic DHT promotes the infiltration of CD8+ T cells in BPH tissues via modulation of CCL5 secretion.
MA5-14548 was used in immunohistochemistry to study the role of CCL5 secretion in the mechanism by which CD8(+) T-cell infiltration of benign prostatic hyperplasia tissue is enhanced by low dihydroxytestosterone levels
|Fan Y,Hu S,Liu J,Xiao F,Li X,Yu W,Cui Y,Sun M,Lv T,He Q,Jin J||Mediators of inflammation (2014:null)||2014|
Prognostic implications of tumor-infiltrating FoxP3+ regulatory T cells and CD8+ cytotoxic T cells in microsatellite-unstable gastric cancers.
MA5-14548 was used in immunohistochemistry to study the prognostic value of FoxP3-positive regulatory T-cells and CD8-positive cytotoxic T-cells in gastric cancers with high microsatellite instability
|Kim KJ,Lee KS,Cho HJ,Kim YH,Yang HK,Kim WH,Kang GH||Human pathology (45:285)||2014|
T cell-activation in neuromyelitis optica lesions plays a role in their formation.
MA5-14548 was used in immunohistochemistry to study the role of T-cell activation in the formation of neuromyelitis optica lesions
|Pohl M,Kawakami N,Kitic M,Bauer J,Martins R,Fischer MT,Machado-Santos J,Mader S,Ellwart JW,Misu T,Fujihara K,Wekerle H,Reindl M,Lassmann H,Bradl M||Acta neuropathologica communications (1:null)||2013|
Expression of CD8 is associated with non-common type morphology and outcome in pediatric anaplastic lymphoma kinase-positive anaplastic large cell lymphoma.
MA5-14548 was used in immunohistochemistry to study the prognostic value of CD8 expression in pediatric anaplastic lymphoma kinase-positive anaplastic large cell lymphoma and its association with non-common type morphology
|Abramov D,Oschlies I,Zimmermann M,Konovalov D,Damm-Welk C,Wössmann W,Klapper W||Haematologica (98:1547)||2013|
Tumor-infiltrating lymphocytes, tumor characteristics, and recurrence in patients with early breast cancer.
MA5-14548 was used in immunohistochemistry to study the relationships between tumor-infiltrating lymphocytes, breast cancer characteristics and tumor recurrence
|Kim ST,Jeong H,Woo OH,Seo JH,Kim A,Lee ES,Shin SW,Kim YH,Kim JS,Park KH||American journal of clinical oncology (36:224)||2013|
Lymphoid tissue damage in HIV-1 infection depletes naïve T cells and limits T cell reconstitution after antiretroviral therapy.
MA5-14548 was used in immunohistochemistry to test if damage to the lymphoid tissue fibroblastic reticular cell network contributes to naive T cell loss during HIV-1 infection
|Zeng M,Southern PJ,Reilly CS,Beilman GJ,Chipman JG,Schacker TW,Haase AT||PLoS pathogens (8:null)||2012|
Anaplastic large cell lymphoma with paraneoplastic leukocytosis: a clinicopathological analysis of five cases.
MA5-14548 was used in immunohistochemistry to report on five cases of anaplastic large cell lymphoma with paraneoplastic leukocytosis
|Chang IW,Chen HK,Ma MC,Huang WT||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (119:794)||2011|
Study of cellular immune response against Hepatitis E virus (HEV).
MA5-14548 was used in immunohistochemistry to investigate the immunopathology of hepatitis E virus infection
|Prabhu SB,Gupta P,Durgapal H,Rath S,Gupta SD,Acharya SK,Panda SK||Journal of viral hepatitis (18:587)||2011|
CD8¿ T cells with an intraepithelial phenotype upregulate cytotoxic function upon influenza infection in human lung.
MA5-14548 was used in immunohistochemistry to identify the role of CD103+CD8+ T cells in protection against respiratory viruses
|Piet B,de Bree GJ,Smids-Dierdorp BS,van der Loos CM,Remmerswaal EB,von der Thüsen JH,van Haarst JM,Eerenberg JP,ten Brinke A,van der Bij W,Timens W,van Lier RA,Jonkers RE||The Journal of clinical investigation (121:2254)||2011|
Spatial differences in the presence of FOXP3+ and GranzymeB+ T cells between the intra- and extravascular compartments in renal allograft vasculopathy.
MA5-14548 was used in immunohistochemistry to investigate the differences of T cell subtypes between the intra- and extravascular compartments in renal allograft vasculopathy
|de Boer OJ,Teeling P,Jansen M,Ploegmakers H,van der Loos CM,Kummer JA,Florquin S,van der Wal AC||PloS one (6:null)||2011|
Hepatitis C virus induces the expression of CCL17 and CCL22 chemokines that attract regulatory T cells to the site of infection.
MA5-14548 was used in immunohistochemistry to study the role of CCL17 and CCL22 in Foxp3+ T regulatory cell accumulation at the site of HCV infection
|Riezu-Boj JI,Larrea E,Aldabe R,Guembe L,Casares N,Galeano E,Echeverria I,Sarobe P,Herrero I,Sangro B,Prieto J,Lasarte JJ||Journal of hepatology (54:422)||2011|
Antagonism of TIM-1 blocks the development of disease in a humanized mouse model of allergic asthma.
MA5-14548 was used in immunohistochemistry to investigate the role of the TIM-1 pathway in T cell activation and allergic asthma
|Sonar SS,Hsu YM,Conrad ML,Majeau GR,Kilic A,Garber E,Gao Y,Nwankwo C,Willer G,Dudda JC,Kim H,Bailly V,Pagenstecher A,Rennert PD,Renz H||The Journal of clinical investigation (120:2767)||2010|
Primary central nervous system peripheral T-cell lymphoma in a child.
MA5-14548 was used in immunohistochemistry to report on a case of primary central nervous system peripheral T-cell lymphoma in a child
|Gualco G,Wludarski S,Hayashi-Silva L,Medeiros Filho P,Veras G,Bacchi CE||Fetal and pediatric pathology (29:224)||2010|
Increased T-bet+ cytotoxic effectors and type I interferon-mediated processes in chronic graft-versus-host disease of the oral mucosa.
MA5-14548 was used in immunohistochemistry to investigate the pathogenesis of chronic graft-versus-host disease
|Imanguli MM,Swaim WD,League SC,Gress RE,Pavletic SZ,Hakim FT||Blood (113:3620)||2009|
GMP-17-positive T-lymphocytes in renal tubules predict progression in early stages of IgA nephropathy.
MA5-14548 was used in immunohistochemistry to investigate the predictive value of GMP-17-positive T-lymphocytes in IgA nephropathy
|van Es LA,de Heer E,Vleming LJ,van der Wal A,Mallat M,Bajema I,Bruijn JA,de Fijter JW||Kidney international (73:1426)||2008|
Selective expansion of influenza A virus-specific T cells in symptomatic human carotid artery atherosclerotic plaques.
MA5-14548 was used in immunohistochemistry to study the role of influenza A virus infection in atherothrombotic disease
|Keller TT,van der Meer JJ,Teeling P,van der Sluijs K,Idu MM,Rimmelzwaan GF,Levi M,van der Wal AC,de Boer OJ||Stroke (39:174)||2008|
Effect of melatonin and zinc on the immune response in experimental Toxoplasma retinochoroiditis.
MA5-14548 was used in immunohistochemistry to investigate the impact of melatonin and zinc on immune and inflammatory responses to Toxoplasma retinochoroiditis in a rat model
|Avunduk AM,Avunduk MC,Baltaci AK,Moğulkoç R||Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fu¿r Augenheilkunde (221:421)||2007|
Intrahepatic virus-specific IL-10-producing CD8 T cells prevent liver damage during chronic hepatitis C virus infection.
MA5-14548 was used in immunohistochemistry to investigate the role of CD8 T cells in the liver damage during chronic hepatitis C virus infection
|Abel M,Sène D,Pol S,Bourlière M,Poynard T,Charlotte F,Cacoub P,Caillat-Zucman S||Hepatology (Baltimore, Md.) (44:1607)||2006|
Nontuberculous mycobacterial infection with concurrent IgG4-related lymphadenopathy.
MA5-14548 was used in immunohistochemistry - paraffin section to study nontuberculous mycobacterial infection with mutual IgG4-related lymphadenopathy
|Liu TT,Weng SW,Wang MC,Huang WT||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (124:216)||2016|
Prognostic Implication of M2 Macrophages Are Determined by the Proportional Balance of Tumor Associated Macrophages and Tumor Infiltrating Lymphocytes in Microsatellite-Unstable Gastric Carcinoma.
MA5-14548 was used in immunohistochemistry - paraffin section to assess how proportional balance of tumor associated macrophages and tumor infiltrating lymphocytes in microsatellite-unstable gastric carcinoma is a prognostic indicator of M2 macrophages
|Kim KJ,Wen XY,Yang HK,Kim WH,Kang GH||PloS one (10:null)||2015|
Tumor-Infiltrating Immune Cells Are Associated With Prognosis of Gastric Cancer.
MA5-14548 was used in immunohistochemistry - paraffin section to elucidate the prognosis of gastric cancer by tumor-infiltrating immune cells
|Liu K,Yang K,Wu B,Chen H,Chen X,Chen X,Jiang L,Ye F,He D,Lu Z,Xue L,Zhang W,Li Q,Zhou Z,Mo X,Hu J||Medicine (94:null)||2015|
CUX1 modulates polarization of tumor-associated macrophages by antagonizing NF-¿B signaling.
MA5-14548 was used in immunocytochemistry to study the role of NFkB in the mechanism by which CUX1 regulates tumor-associated macrophage functionality
|Kühnemuth B,Mühlberg L,Schipper M,Griesmann H,Neesse A,Milosevic N,Wissniowski T,Buchholz M,Gress TM,Michl P||Oncogene (34:177)||2015|