|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminus of alpha chain of the human CD8 molecule|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
CD8 molecule consists of two chains, termed alpha and beta chain, which are expressed as a disulphide-linked alpha/beta heterdimer or as an alpha/alpha homodimer on T cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as alpha/beta heterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 alpha chain (but not beta chain).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nontuberculous mycobacterial infection with concurrent IgG4-related lymphadenopathy.
MA5-16345 was used in immunohistochemistry - paraffin section to study nontuberculous mycobacterial infection with mutual IgG4-related lymphadenopathy
|Liu TT,Weng SW,Wang MC,Huang WT||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (124:216)||2016|
Prognostic Implication of M2 Macrophages Are Determined by the Proportional Balance of Tumor Associated Macrophages and Tumor Infiltrating Lymphocytes in Microsatellite-Unstable Gastric Carcinoma.
MA5-16345 was used in immunohistochemistry - paraffin section to assess how proportional balance of tumor associated macrophages and tumor infiltrating lymphocytes in microsatellite-unstable gastric carcinoma is a prognostic indicator of M2 macrophages
|Kim KJ,Wen XY,Yang HK,Kim WH,Kang GH||PloS one (10:null)||2015|
Tumor-Infiltrating Immune Cells Are Associated With Prognosis of Gastric Cancer.
MA5-16345 was used in immunohistochemistry - paraffin section to elucidate the prognosis of gastric cancer by tumor-infiltrating immune cells
|Liu K,Yang K,Wu B,Chen H,Chen X,Chen X,Jiang L,Ye F,He D,Lu Z,Xue L,Zhang W,Li Q,Zhou Z,Mo X,Hu J||Medicine (94:null)||2015|
CUX1 modulates polarization of tumor-associated macrophages by antagonizing NF-¿B signaling.
MA5-16345 was used in immunocytochemistry to study the role of NFkB in the mechanism by which CUX1 regulates tumor-associated macrophage functionality
|Kühnemuth B,Mühlberg L,Schipper M,Griesmann H,Neesse A,Milosevic N,Wissniowski T,Buchholz M,Gress TM,Michl P||Oncogene (34:177)||2015|
Low intraprostatic DHT promotes the infiltration of CD8+ T cells in BPH tissues via modulation of CCL5 secretion.
MA5-16345 was used in immunohistochemistry to study the role of CCL5 secretion in the mechanism by which CD8(+) T-cell infiltration of benign prostatic hyperplasia tissue is enhanced by low dihydroxytestosterone levels
|Fan Y,Hu S,Liu J,Xiao F,Li X,Yu W,Cui Y,Sun M,Lv T,He Q,Jin J||Mediators of inflammation (2014:null)||2014|
Prognostic implications of tumor-infiltrating FoxP3+ regulatory T cells and CD8+ cytotoxic T cells in microsatellite-unstable gastric cancers.
MA5-16345 was used in immunohistochemistry to study the prognostic value of FoxP3-positive regulatory T-cells and CD8-positive cytotoxic T-cells in gastric cancers with high microsatellite instability
|Kim KJ,Lee KS,Cho HJ,Kim YH,Yang HK,Kim WH,Kang GH||Human pathology (45:285)||2014|
T cell-activation in neuromyelitis optica lesions plays a role in their formation.
MA5-16345 was used in immunohistochemistry to study the role of T-cell activation in the formation of neuromyelitis optica lesions
|Pohl M,Kawakami N,Kitic M,Bauer J,Martins R,Fischer MT,Machado-Santos J,Mader S,Ellwart JW,Misu T,Fujihara K,Wekerle H,Reindl M,Lassmann H,Bradl M||Acta neuropathologica communications (1:null)||2013|
Expression of CD8 is associated with non-common type morphology and outcome in pediatric anaplastic lymphoma kinase-positive anaplastic large cell lymphoma.
MA5-16345 was used in immunohistochemistry to study the prognostic value of CD8 expression in pediatric anaplastic lymphoma kinase-positive anaplastic large cell lymphoma and its association with non-common type morphology
|Abramov D,Oschlies I,Zimmermann M,Konovalov D,Damm-Welk C,Wössmann W,Klapper W||Haematologica (98:1547)||2013|
Tumor-infiltrating lymphocytes, tumor characteristics, and recurrence in patients with early breast cancer.
MA5-16345 was used in immunohistochemistry to study the relationships between tumor-infiltrating lymphocytes, breast cancer characteristics and tumor recurrence
|Kim ST,Jeong H,Woo OH,Seo JH,Kim A,Lee ES,Shin SW,Kim YH,Kim JS,Park KH||American journal of clinical oncology (36:224)||2013|
Anaplastic large cell lymphoma with paraneoplastic leukocytosis: a clinicopathological analysis of five cases.
MA5-16345 was used in immunohistochemistry to report on five cases of anaplastic large cell lymphoma with paraneoplastic leukocytosis
|Chang IW,Chen HK,Ma MC,Huang WT||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (119:794)||2011|
Hepatitis C virus induces the expression of CCL17 and CCL22 chemokines that attract regulatory T cells to the site of infection.
MA5-16345 was used in immunohistochemistry to study the role of CCL17 and CCL22 in Foxp3+ T regulatory cell accumulation at the site of HCV infection
|Riezu-Boj JI,Larrea E,Aldabe R,Guembe L,Casares N,Galeano E,Echeverria I,Sarobe P,Herrero I,Sangro B,Prieto J,Lasarte JJ||Journal of hepatology (54:422)||2011|
Primary central nervous system peripheral T-cell lymphoma in a child.
MA5-16345 was used in immunohistochemistry to report on a case of primary central nervous system peripheral T-cell lymphoma in a child
|Gualco G,Wludarski S,Hayashi-Silva L,Medeiros Filho P,Veras G,Bacchi CE||Fetal and pediatric pathology (29:224)||2010|