|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Storage buffer||PBS, pH 7.4, with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Two alternative transcripts encoding distinct isoforms, one membrane associated and one secreted, have been identified [Gene Wiki: CD8a].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fowlpox-based survivin vaccination for malignant mesothelioma therapy.
MA1-82111 was used in immunohistochemistry to study the therapeutic potential of a novel Fowlpox-based survivin vaccination in a murine model of malignant mesothelioma
|Bertino P,Panigada M,Soprana E,Bianchi V,Bertilaccio S,Sanvito F,Rose AH,Yang H,Gaudino G,Hoffmann PR,Siccardi A,Carbone M||International journal of cancer (133:612)||2013|
Extensive proliferation of T cell lineage-restricted progenitors in the thymus: an essential process for clonal expression of diverse T cell receptor beta chains.
MA1-82111 was used in flow cytometry to assess the proliferative potential of thymic progenitors that are T cell lineage restricted.
|Kawamoto H,Ohmura K,Fujimoto S,Lu M,Ikawa T,Katsura Y||European journal of immunology (33:606)||2003|
IL-18 enhances IL-4 production by ligand-activated NKT lymphocytes: a pro-Th2 effect of IL-18 exerted through NKT cells.
MA1-82111 was used in flow cytometry to investigate the effect of IL-18 on NKT cells.
|Leite-De-Moraes MC,Hameg A,Pacilio M,Koezuka Y,Taniguchi M,Van Kaer L,Schneider E,Dy M,Herbelin A||Journal of immunology (Baltimore, Md. : 1950) (166:945)||2001|
Memory CD44(int) CD8 T cells show increased proliferative responses and IFN-gamma production following antigenic challenge in vitro.
MA1-82111 was used in flow cytometry to investigate hyper responsive CD8 T cells.
|Pihlgren M,Arpin C,Walzer T,Tomkowiak M,Thomas A,Marvel J,Dubois PM||International immunology (11:699)||1999|
Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells.
MA1-82111 was used in flow cytometry to elucidate the pathway and cytokine regulation of NK cell development in vitro.
|Aiba Y,Hirayama F,Ogawa M||Blood (89:4005)||1997|
Resting memory CD8+ T cells are hyperreactive to antigenic challenge in vitro.
MA1-82111 was used in flow cytometry to test if different activation thresholds distinguish naive from memory CD8+ T cell populations.
|Pihlgren M,Dubois PM,Tomkowiak M,Sjögren T,Marvel J||The Journal of experimental medicine (184:2141)||1996|
Helper T cells without CD4: control of leishmaniasis in CD4-deficient mice.
MA1-82111 was used in flow cytometry to suggest that T lymphocyte lineage commitment and peripheral function does not depend on functional CD4.
|Locksley RM,Reiner SL,Hatam F,Littman DR,Killeen N||Science (New York, N.Y.) (261:1448)||1993|
Binding of the integrin Mac-1 (CD11b/CD18) to the third immunoglobulin-like domain of ICAM-1 (CD54) and its regulation by glycosylation.
MA1-82111 was used in ELISA and flow cytometry to compare the domains needed for LFA-1 and Mac-1 to bind ICAM-1.
|Diamond MS,Staunton DE,Marlin SD,Springer TA||Cell (65:961)||1991|