|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1/IgG2a|
|Immunogen||human peripheral blood T lymphocytes|
|Conjugate||FITC , PE|
|Storage buffer||PBS with 0.2% carrier protein|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is a monoclonal mixture of FITC-conjugated CD8 (clone 17D8, isotype IgG1) and PE-conjugated CD11a (clone OFYS, isotype IgG2a).
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. CD11a is a glycoprotein with a molecular weight of 26 kDa expressed in combination with the CD18 beta chain. The complex is a member of the beta2 integrin family. These molecules function in cell adhesion and specifically bind to CD54, ICAM-2, ICAM-3. CD11a is expressed on lymphocytes, granulocytes, monocytes, and macrophages and is upregulated on activated T-cells. Anti-CD11a from I21/7 reacts with 97% of thymocytes, 95% of blood lymphocytes, 70% of bone marrow cells of all mouse strains tested.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.