|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Con A-stimulated BALB/c splenic T cells|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hydrophobic CDR3 residues promote the development of self-reactive T cells.
MCD0817 was used in flow cytometry to study the promotion of the development of self-reactive T cells by hydrophobic CDR3 residues
|Stadinski BD,Shekhar K,Gómez-Touriño I,Jung J,Sasaki K,Sewell AK,Peakman M,Chakraborty AK,Huseby ES||Nature immunology (17:946)||2016|
|Not Applicable||Not Cited||
Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii.
MCD0817 was used in flow cytometry to study interactions between CD8 T cells, Toxoplasma gondii, and APCs in the brain
|Schaeffer M,Han SJ,Chtanova T,van Dooren GG,Herzmark P,Chen Y,Roysam B,Striepen B,Robey EA||Journal of immunology (Baltimore, Md. : 1950) (182:6379)||2009|
|Not Applicable||Not Cited||
Hematopoietic stem cell dose correlates with the speed of immune reconstitution after stem cell transplantation.
MCD0817 was used in flow cytometry to test if higher numbers of hematopoietic stem cells correlate with the speed of immune reconstitution in a congenic transplantation mouse model
|Chen BJ,Cui X,Sempowski GD,Domen J,Chao NJ||Blood (103:4344)||2004|
A spread-deficient cytomegalovirus for assessment of first-target cells in vaccination.
MCD0817 was used in flow cytometry to characterize a spread-deficient murine cytomegalovirus as a novel approach for betaherpesvirus vaccination
|Mohr CA,Arapovic J,Mühlbach H,Panzer M,Weyn A,Dölken L,Krmpotic A,Voehringer D,Ruzsics Z,Koszinowski U,Sacher T||Journal of virology (84:7730)||2010|
Marked inhibition of transplant vascular sclerosis by in vivo-mobilized donor dendritic cells and anti-CD154 mAb.
MCD0817 was used in flow cytometry to determine the influence of in vivo-mobilized immature donor DC plus anti-CD40L mAb on vascular sclerosis in functional murine aortic allografts.
|Wang Z,Morelli AE,Hackstein H,Kaneko K,Thomson AW||Transplantation (76:562)||2003|