|Tested species reactivity||Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||T cell clone, C6|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100 -1:200|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD8 is expressed on T-cells and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cel-to-cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules. CD8 alpha is expressed by a subset of T lymphocytes, most thymocytes, and the majority of NK cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of adaptive immunity by IL9 can be disrupted to achieve rapid T-cell sensitization and rejection of progressive tumor challenges.
MA5-16761 was used in immunohistochemistry - paraffin section to study the effects of IL9 in multiple models of breast and colon cancer development using IL-9 KO mice
|Hoelzinger DB,Dominguez AL,Cohen PA,Gendler SJ||Cancer research (74:6845)||2014|
Gene transfer of the S24F regulated on activation normal T-cell expressed and secreted-chemokine ligand 5 variant attenuates cardiac allograft rejection.
MA5-16761 was used in immunohistochemistry to study the reduction in cardiac allograft rejection mediated by adenoviral expression of the naturally occurring S24F variant of RANTES
|Sun Y,Feng J,Shi J,Lu X,Xie A,Chen Y,Dong N||Transplantation (97:1233)||2014|
|Not Applicable||Not Cited||
Protection of non-obese diabetic mice from autoimmune diabetes by Escherichia coli heat-labile enterotoxin B subunit.
MA5-16761 was used in immunohistochemistry to show that EtxB can block diabetes
|Ola TO,Williams NA||Immunology (117:262)||2006|