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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Mouse Ly-2 thymocytes|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1 ug/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 10 publications below|
MA5-17604 targets CD8a/T subset NK in FACS applications and shows reactivity with Mouse samples.
The MA5-17604 immunogen is mouse Ly-2 thymocytes.
This antibody recognizes CD8. CD8 is composed of an alpha chain with Mr=30-34 kDa linked by disulfide bonds to a beta chain with Mr=30-32 kDa. In addition to forming heterodimers with the beta chain, the alpha chain is also capable of forming homodimers while the beta chain is incapable of forming homodimers. The extracellular portion of CD8alpha binds to the non-polymorphic portion of MHC class I molecules. This interaction is important in the antigen presentation to CD8+ T cells. The cytoplasmic domain of the alpha chain associates with p56lck, a member of the Src family of tyrosine kinases. Cells which are reactive with this antibody include: thymocytes, natural killer cells, and a subset (CD4-) of peripheral T-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Extensive proliferation of T cell lineage-restricted progenitors in the thymus: an essential process for clonal expression of diverse T cell receptor beta chains.
MA5-17604 was used in flow cytometry to assess the proliferative potential of thymic progenitors that are T cell lineage restricted.
|Kawamoto H,Ohmura K,Fujimoto S,Lu M,Ikawa T,Katsura Y||European journal of immunology (33:606)||2003|
Immature multipotent hemopoietic progenitors lacking long-term bone marrow-reconstituting activity in the aorta-gonad-mesonephros region of murine day 10 fetuses.
MA5-17604 was used in flow cytometry to examine multipotent progenitors from early fetuses.
|Ohmura K,Kawamoto H,Lu M,Ikawa T,Ozaki S,Nakao K,Katsura Y||Journal of immunology (Baltimore, Md. : 1950) (166:3290)||2001|
IL-18 enhances IL-4 production by ligand-activated NKT lymphocytes: a pro-Th2 effect of IL-18 exerted through NKT cells.
MA5-17604 was used in flow cytometry to investigate the effect of IL-18 on NKT cells.
|Leite-De-Moraes MC,Hameg A,Pacilio M,Koezuka Y,Taniguchi M,Van Kaer L,Schneider E,Dy M,Herbelin A||Journal of immunology (Baltimore, Md. : 1950) (166:945)||2001|
Commitment of common T/Natural killer (NK) progenitors to unipotent T and NK progenitors in the murine fetal thymus revealed by a single progenitor assay.
MA5-17604 was used in flow cytometry to describe a clonal system that supports the development of T and natural killer cells evenly.
|Ikawa T,Kawamoto H,Fujimoto S,Katsura Y||The Journal of experimental medicine (190:1617)||1999|
Emergence of T, B, and myeloid lineage-committed as well as multipotent hemopoietic progenitors in the aorta-gonad-mesonephros region of day 10 fetuses of the mouse.
MA5-17604 was used in flow cytometry to investigate the aorta-gonad-mesonephros region as a source of hemopoietic progenitors.
|Ohmura K,Kawamoto H,Fujimoto S,Ozaki S,Nakao K,Katsura Y||Journal of immunology (Baltimore, Md. : 1950) (163:4788)||1999|
Memory CD44(int) CD8 T cells show increased proliferative responses and IFN-gamma production following antigenic challenge in vitro.
MA5-17604 was used in flow cytometry to investigate hyper responsive CD8 T cells.
|Pihlgren M,Arpin C,Walzer T,Tomkowiak M,Thomas A,Marvel J,Dubois PM||International immunology (11:699)||1999|
Emergence of T cell progenitors without B cell or myeloid differentiation potential at the earliest stage of hematopoiesis in the murine fetal liver.
MA5-17604 was used in flow cytometry to assess if progenitors colonizing the thymus are T cell lineage restricted.
|Kawamoto H,Ohmura K,Fujimoto S,Katsura Y||Journal of immunology (Baltimore, Md. : 1950) (162:2725)||1999|
Isolation of the most immature population of murine fetal thymocytes that includes progenitors capable of generating T, B, and myeloid cells.
MA5-17604 was used in flow cytometry to report that FcR is a useful marker to isolate the most immature population of murine fetal thymic cells.
|Hattori N,Kawamoto H,Katsura Y||The Journal of experimental medicine (184:1901)||1996|
Exclusive development of T cell neoplasms in mice transplanted with bone marrow expressing activated Notch alleles.
MA5-17604 was used in flow cytometry to report that TAN1 is an oncoprotein.
|Pear WS,Aster JC,Scott ML,Hasserjian RP,Soffer B,Sklar J,Baltimore D||The Journal of experimental medicine (183:2283)||1996|
CD3+ and CD4+ cells adoptively transfer experimental hypersensitivity pneumonitis.
MA5-17604 was used in flow cytometry to describe the cells that transfer adoptive murine experimental hypersensitivity pneumonitis.
|Schuyler M,Gott K,Shopp G,Crooks L||The American review of respiratory disease (146:1582)||1992|
CD8; CD8a; Leu-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); MAL; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2
BB154331; Cd8a; Ly-2; Ly-35; Ly-B; Lyt-2; Lyt2