|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.0 ug/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 7 publications below|
MA5-17606 targets CD8a/T subset NK in FACS applications and shows reactivity with Mouse samples.
The MA5-17606 immunogen is mouse thymocytes.
This antibody recognizes CD8. CD8 is composed of an alpha chain with Mr=30-34 kDa linked by disulfide bonds to a beta chain with Mr=30-32 kDa. In addition to forming heterodimers with the beta chain, the alpha chain is also capable of forming homodimers while the beta chain is incapable of forming homodimers. The extracellular portion of CD8alpha binds to the non-polymorphic portion of MHC class I molecules. This interaction is important in the antigen presentation to CD8+ T cells. The cytoplasmic domain of the alpha chain associates with p56lck, a member of the Src family of tyrosine kinases. Cells which are reactive with this antibody include: thymocytes, natural killer cells, and a subset (CD4-) of peripheral T-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Extensive proliferation of T cell lineage-restricted progenitors in the thymus: an essential process for clonal expression of diverse T cell receptor beta chains.
MA5-17606 was used in flow cytometry to assess the proliferative potential of thymic progenitors that are T cell lineage restricted.
|Kawamoto H,Ohmura K,Fujimoto S,Lu M,Ikawa T,Katsura Y||European journal of immunology (33:606)||2003|
IL-18 enhances IL-4 production by ligand-activated NKT lymphocytes: a pro-Th2 effect of IL-18 exerted through NKT cells.
MA5-17606 was used in flow cytometry to investigate the effect of IL-18 on NKT cells.
|Leite-De-Moraes MC,Hameg A,Pacilio M,Koezuka Y,Taniguchi M,Van Kaer L,Schneider E,Dy M,Herbelin A||Journal of immunology (Baltimore, Md. : 1950) (166:945)||2001|
Memory CD44(int) CD8 T cells show increased proliferative responses and IFN-gamma production following antigenic challenge in vitro.
MA5-17606 was used in flow cytometry to investigate hyper responsive CD8 T cells.
|Pihlgren M,Arpin C,Walzer T,Tomkowiak M,Thomas A,Marvel J,Dubois PM||International immunology (11:699)||1999|
Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells.
MA5-17606 was used in flow cytometry to elucidate the pathway and cytokine regulation of NK cell development in vitro.
|Aiba Y,Hirayama F,Ogawa M||Blood (89:4005)||1997|
Resting memory CD8+ T cells are hyperreactive to antigenic challenge in vitro.
MA5-17606 was used in flow cytometry to test if different activation thresholds distinguish naive from memory CD8+ T cell populations.
|Pihlgren M,Dubois PM,Tomkowiak M,Sjögren T,Marvel J||The Journal of experimental medicine (184:2141)||1996|
Helper T cells without CD4: control of leishmaniasis in CD4-deficient mice.
MA5-17606 was used in flow cytometry to suggest that T lymphocyte lineage commitment and peripheral function does not depend on functional CD4.
|Locksley RM,Reiner SL,Hatam F,Littman DR,Killeen N||Science (New York, N.Y.) (261:1448)||1993|
Binding of the integrin Mac-1 (CD11b/CD18) to the third immunoglobulin-like domain of ICAM-1 (CD54) and its regulation by glycosylation.
MA5-17606 was used in ELISA and flow cytometry to compare the domains needed for LFA-1 and Mac-1 to bind ICAM-1.
|Diamond MS,Staunton DE,Marlin SD,Springer TA||Cell (65:961)||1991|