|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Mouse B7-1 protein.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/1 million cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-70090 detects CD80 in mouse samples.
MA1-70090 has been successfully used in flow cytometry procedures.
The MA1-70090 immunogen is mouse B7-1 protein.
CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Dendritic cell modification as a route to inhibiting corneal graft rejection by the indirect pathway of allorecognition.
MA1-70090 was used in flow cytometry to compare two dendritic cell modification strategies to inhibit allogeneic T-cell proliferation
|Khan A,Fu H,Tan LA,Harper JE,Beutelspacher SC,Larkin DF,Lombardi G,McClure MO,George AJ||European journal of immunology (43:734)||2013|
|Not Applicable||Not Cited||
Dendritic cells activated with products released by schistosome larvae drive Th2-type immune responses, which can be inhibited by manipulation of CD40 costimulation.
MA1-70090 was used in flow cytometry to investigate how products released by schistosome larvae affect dendritic cells
|Jenkins SJ,Mountford AP||Infection and immunity (73:395)||2005|
Activation of dendritic cells and induction of T cell responses by HPV 16 L1/E7 chimeric virus-like particles are enhanced by CpG ODN or sorbitol.
MA1-70090 was used in flow cytometry to study the interaction of human papillomavirus 16 L1/E7 chimeric virus-like particles with mouse bone marrow-derived dendritic cells activated with different immune adjuvants
|Freyschmidt EJ,Alonso A,Hartmann G,Gissmann L||Antiviral therapy (9:479)||2004|
Passive transfer of flt-3L-derived dendritic cells delays diabetes development in NOD mice and associates with early production of interleukin (IL)-4 and IL-10 in the spleen of recipient mice.
MA1-70090 was used in flow cytometry to assess the contribution of DC function in non-obese diabetic mice.
|Morin J,Faideau B,Gagnerault MC,Lepault F,Boitard C,Boudaly S||Clinical and experimental immunology (134:388)||2003|
Naive B lymphocytes undergo homeostatic proliferation in response to B cell deficit.
MA1-70090 was used in flow cytometry to investigate homeostatic B cell proliferation.
|Cabatingan MS,Schmidt MR,Sen R,Woodland RT||Journal of immunology (Baltimore, Md. : 1950) (169:6795)||2002|
Macrophage C-type lectin on bone marrow-derived immature dendritic cells is involved in the internalization of glycosylated antigens.
MA1-70090 was used in flow cytometry to study expression of a C-type lectin specific for galactose and N-acetylgalactosamine on murine dendritic cells.
|Denda-Nagai K,Kubota N,Tsuiji M,Kamata M,Irimura T||Glycobiology (12:443)||2002|
Activation of MHC class I, II, and CD40 gene expression by histone deacetylase inhibitors.
MA1-70090 was used in flow cytometry to report that histone deacetylase inhibitors alter the acetylation of histones and enhance the expression of several genes on tumor cells.
|Magner WJ,Kazim AL,Stewart C,Romano MA,Catalano G,Grande C,Keiser N,Santaniello F,Tomasi TB||Journal of immunology (Baltimore, Md. : 1950) (165:7017)||2000|