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Invitrogen
CD80 (B7-1) Monoclonal Antibody (3H5), PE, eBioscience™
Product Details
12-0800-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
3H5
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_465749
Product Specific Information
Description: The 3H5 monoclonal antibody reacts with rat CD80 (B7-1), an ~70 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. 3H5 blocks binding of CD80 to its ligands.^M
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Applications Reported: The 3H5 antibody has been reported for use in flow cytometric analysis.^M
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Applications Tested: The 3H5 antibody has been tested by flow cytometric analysis of activated rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.^M
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Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.^M
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Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 antigen; sCD 80; sCD80; soluble CD 80; soluble CD80; T-lymphocyte activation antigen CD80; TS/A-1
Gene Aliases: B7-1
Entrez Gene ID: (Rat) 25408
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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