|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human peripheral blood lymphocytes.|
|Storage buffer||PBS, pH 7.2, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Major histocompatibility complex class II (DR) antigen and costimulatory molecules on in vitro and in vivo activated human polymorphonuclear neutrophils.
MA1-81186 was used in flow cytometry and immunocytochemistry to compare X-L of Mac -1 with other neutrophil activators
|Sandilands GP,McCrae J,Hill K,Perry M,Baxter D||Immunology (119:562)||2006|
|Not Applicable||3.3 µg/ml||
Profiling dendritic cell maturation with dedicated microarrays.
MA1-81186 was used in flow cytometry to develop a dendritic cell-dedicated microarray
|McIlroy D,Tanguy-Royer S,Le Meur N,Guisle I,Royer PJ,Léger J,Meflah K,Grégoire M||Journal of leukocyte biology (78:794)||2005|
|Not Applicable||Not Cited||
Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions.
MA1-81186 was used in flow cytometry to report that OX40 ligand is selectively induced by IL-2, IL-12, or IL-15-activated human NK cells following stimulation through NKG2D
|Zingoni A,Sornasse T,Cocks BG,Tanaka Y,Santoni A,Lanier LL||Journal of immunology (Baltimore, Md. : 1950) (173:3716)||2004|
Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.
MA1-81186 was used in flow cytometry to report that CD34(+) progenitor cell-derived Langerhans cells-type DCs exhibit a differentiation state-dependent susceptibility to CMV infection.
|Hertel L,Lacaille VG,Strobl H,Mellins ED,Mocarski ES||Journal of virology (77:7563)||2003|
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
MA1-81186 was used in flow cytometry to elucidate the importance of C domains in the functioning of CD80 and CD86.
|Vasu C,Wang A,Gorla SR,Kaithamana S,Prabhakar BS,Holterman MJ||International immunology (15:167)||2003|
CD40 ligand-stimulated B cell precursor leukemic cells elicit interferon-gamma production by autologous bone marrow T cells in childhood acute lymphoblastic leukemia.
MA1-81186 was used in flow cytometry to test if CD40L activates the antigen-presenting cell capacity of B cell precursor acute lymphoblastic leukemia cells.
|Todisco E,Gaipa G,Biagi E,Bonamino M,Gramigna R,Introna M,Biondi A||Leukemia (16:2046)||2002|
Human CD4(+)CD25(+) cells: a naturally occurring population of regulatory T cells.
MA1-81186 was used in flow cytometry to characterize CD4(+)CD25(+) human T cells.
|Ng WF,Duggan PJ,Ponchel F,Matarese G,Lombardi G,Edwards AD,Isaacs JD,Lechler RI||Blood (98:2736)||2001|