Invitrogen

CD86 (B7-2) Monoclonal Antibody (GL1), PE-Cyanine5, eBioscience™

Product Image Gallery

CD86 (B7-2) Antibody in Flow Cytometry (Flow) — Staining of 3-day LPS activated BALB/c splenocytes with 0.03 µg of Rat IgG2a Isotype Control PE-Cyanine5 (Product # 15-4321-80) (open histogram) or Anti-Mouse CD86 (B7-2) PE-Cyanine5 (filled histogram).Total viable cells were used for analysis.

Product Details

15-0862-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.06 µg/test	View 41 publications 41 publications

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	GL1
Conjugate	PE-Cyanine5 Alexa Fluor 700 APC APC-eFluor 780 Biotin eFluor 450 FITC PE PE-Cyanine7 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_468778

Product Specific Information

Description: The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.

Applications Reported: The GL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The GL1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury.

Frontiers in cellular neuroscience

Hellström Erkenstam N,Smith PL,Fleiss B,Nair S,Svedin P,Wang W,Boström M,Gressens P,Hagberg H,Brown KL,Sävman K,Mallard C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

MiR-103 protects from recurrent spontaneous abortion via inhibiting STAT1 mediated M1 macrophage polarization.

International journal of biological sciences

Zhu X,Liu H,Zhang Z,Wei R,Zhou X,Wang Z,Zhao L,Guo Q,Zhang Y,Chu C,Wang L,Li X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Fri Nov 12 00:00:00 EST 2021

Adrenomedullin 2 improves bone regeneration in type 1 diabetic rats by restoring imbalanced macrophage polarization and impaired osteogenesis.

Stem cell research & therapy

Wang F,Kong L,Wang W,Shi L,Wang M,Chai Y,Xu J,Kang Q

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Protein Tyrosine Phosphatase Non-Receptor Type 2 Function in Dendritic Cells Is Crucial to Maintain Tissue Tolerance.

Frontiers in immunology

Hering L,Katkeviciute E,Schwarzfischer M,Busenhart P,Gottier C,Mrdjen D,Komuczki J,Wawrzyniak M,Lang S,Atrott K,Becher B,Rogler G,Scharl M,Spalinger MR

15-0862 was used in Flow cytometry/Cell sorting to show dendritic cell PTPN2 might explain the central role for PTPN2 in the immune system to maintain immune tolerance.

Mon Apr 12 00:00:00 EDT 2021

Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Restructures the Immune Contexture to Improve Checkpoint Blockade Efficacy.

Biomedicines

Ebelt ND,Zuniga E,Marzagalli M,Zamloot V,Blazar BR,Salgia R,Manuel ER

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Mutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction.

Frontiers in immunology

Fenninger F,Han J,Stanwood SR,Nohara LL,Arora H,Choi KB,Munro L,Pfeifer CG,Shanina I,Horwitz MS,Jefferies WA

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

Ginsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype.

Frontiers in pharmacology

Guo M,Xiao J,Sheng X,Zhang X,Tie Y,Wang L,Zhao L,Ji X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

15-0862 was used in Flow cytometry/Cell sorting to study the HDAC inhibitor CG-745 and its function to enable the synergistic effects of the immune checkpoint inhibitor combination therapy in various cancers by suppressing tumor microenvironment.

Mon Sep 28 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

FcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells.

Frontiers in immunology

Pan YG,Yu YL,Lin CC,Lanier LL,Chu CL

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.

Journal of nanobiotechnology

Yuan SJ,Xu YH,Wang C,An HC,Xu HZ,Li K,Komatsu N,Zhao L,Chen X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Thu Oct 17 00:00:00 EDT 2019

Arp2/3 complex-driven spatial patterning of the BCR enhances immune synapse formation, BCR signaling and B cell activation.

eLife

Bolger-Munro M,Choi K,Scurll JM,Abraham L,Chappell RS,Sheen D,Dang-Lawson M,Wu X,Priatel JJ,Coombs D,Hammer JA,Gold MR

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Mon Jun 03 00:00:00 EDT 2019

cGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation Following Mycobacterium bovis Infection.

International journal of molecular sciences

Li Q,Liu C,Yue R,El-Ashram S,Wang J,He X,Zhao D,Zhou X,Xu L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Tue Feb 19 00:00:00 EST 2019

Efficient oral vaccination by bioengineering virus-like particles with protozoan surface proteins.

Nature communications

Serradell MC,Rupil LL,Martino RA,Prucca CG,Carranza PG,Saura A,Fernández EA,Gargantini PR,Tenaglia AH,Petiti JP,Tonelli RR,Reinoso-Vizcaino N,Echenique J,Berod L,Piaggio E,Bellier B,Sparwasser T,Klatzmann D,Luján HD

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Mon Jan 21 00:00:00 EST 2019

Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells.

Stem cells and development

Fujii S,Yoshida S,Inagaki E,Hatou S,Tsubota K,Takahashi M,Shimmura S,Sugita S

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Targeting macrophage and microglia activation with colony stimulating factor 1 receptor inhibitor is an effective strategy to treat injury-triggered neuropathic pain.

Molecular pain

Lee S,Shi XQ,Fan A,West B,Zhang J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Mon Oct 22 00:00:00 EDT 2018

T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells.

Nature communications

Kim HR,Mun Y,Lee KS,Park YJ,Park JS,Park JH,Jeon BN,Kim CH,Jun Y,Hyun YM,Kim M,Lee SM,Park CS,Im SH,Jun CD

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2018

Discrimination of the heterogeneity of bone marrow‑derived dendritic cells.

Molecular medicine reports

Wang J,Dai X,Hsu C,Ming C,He Y,Zhang J,Wei L,Zhou P,Wang CY,Yang J,Gong N

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Calreticulin is an effective immunologic adjuvant to tumor-associated antigens.

Experimental and therapeutic medicine

Wang J,Gao ZP,Qin S,Liu CB,Zou LL

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

IRF6 Regulates Alternative Activation by Suppressing PPARγ in Male Murine Macrophages.

Endocrinology

Li C,Ying W,Huang Z,Brehm T,Morin A,Vella AT,Zhou B

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Metformin Inhibits Advanced Glycation End Products-Induced Inflammatory Response in Murine Macrophages Partly through AMPK Activation and RAGE/NFκB Pathway Suppression.

Journal of diabetes research

Zhou Z,Tang Y,Jin X,Chen C,Lu Y,Liu L,Shen C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Tue Jun 06 00:00:00 EDT 2017

Repulsive guidance molecule a blockade exerts the immunoregulatory function in DCs stimulated with ABP and LPS.

Human vaccines & immunotherapeutics

Xu X,Gao Y,Zhai Z,Zhang S,Shan F,Feng J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Tue Aug 02 00:00:00 EDT 2016

D-Alanylation of Teichoic Acids and Loss of Poly-N-Acetyl Glucosamine in Staphylococcus aureus during Exponential Growth Phase Enhance IL-12 Production in Murine Dendritic Cells.

PloS one

Lund LD,Ingmer H,Frøkiær H

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Thu Jul 14 00:00:00 EDT 2016

CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production in asthmatic mice.

Experimental and therapeutic medicine

Li JG,DU YM,Yan ZD,Yan J,Zhuansun YX,Chen R,Zhang W,Feng SL,Ran PX

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Reducing progression of experimental lupus nephritis via inhibition of the B7/CD28 signaling pathway.

Molecular medicine reports

Huang L,Kong Y,Wang J,Sun J,Shi Q,Qiu YH

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Injectable cryogel-based whole-cell cancer vaccines.

Nature communications

Bencherif SA,Warren Sands R,Ali OA,Li WA,Lewin SA,Braschler TM,Shih TY,Verbeke CS,Bhatta D,Dranoff G,Mooney DJ

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Wed Aug 12 00:00:00 EDT 2015

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy.

Nature biotechnology

Kim J,Li WA,Choi Y,Lewin SA,Verbeke CS,Dranoff G,Mooney DJ

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Lung dendritic cells undergo maturation and polarization towards a T helper type 2-stimulating phenotype in a mouse model of asthma: Role of nerve growth factor.

Experimental and therapeutic medicine

Qin Q,Wang Z,Pan P,Cao Z,Xia Q,Tan H,Hu C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Sat Nov 01 00:00:00 EDT 2014

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Histone deacetylase inhibitors suppress immune activation in primary mouse microglia.

Journal of neuroscience research

Kannan V,Brouwer N,Hanisch UK,Regen T,Eggen BJ,Boddeke HW

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2013

Neutralization of TSLP inhibits airway remodeling in a murine model of allergic asthma induced by chronic exposure to house dust mite.

PloS one

Chen ZG,Zhang TT,Li HT,Chen FH,Zou XL,Ji JZ,Chen H

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Tue Aug 20 00:00:00 EDT 2013

Prolonged apoptotic cell accumulation in germinal centers of Mer-deficient mice causes elevated B cell and CD4+ Th cell responses leading to autoantibody production.

Journal of immunology (Baltimore, Md. : 1950)

Khan TN,Wong EB,Soni C,Rahman ZS

15-0862 was used in Flow cytometry/Cell sorting to study the effect of Mer receptor tyrosine kinase deficiency on the clearance of apoptotic cells.

Fri Feb 15 00:00:00 EST 2013

The lupus-prone NZM2410/NZW strain-derived Sle1b sublocus alters the germinal center checkpoint in female mice in a B cell-intrinsic manner.

Journal of immunology (Baltimore, Md. : 1950)

Wong EB,Khan TN,Mohan C,Rahman ZS

15-0862 was used in Flow cytometry/Cell sorting to show that the effect of Sle1b on Spt-GC, follicular Th cell, and autoantibody responses in B6.Sle1b mice was B cell autonomous.

Sat Dec 15 00:00:00 EST 2012

Immunogenicity and protective capacity of a virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice.

PloS one

Kamphuis T,Meijerhof T,Stegmann T,Lederhofer J,Wilschut J,de Haan A

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Wed Sep 19 00:00:00 EDT 2012

TLR9 and MyD88 are crucial for the development of protective immunity to malaria.

Journal of immunology (Baltimore, Md. : 1950)

Gowda NM,Wu X,Gowda DC

15-0862 was used in Flow cytometry/Cell sorting to investigate TLR9 and MyD88 regulation of pro/anti-inflammatory cytokines in mice infected with Plasmodium yoelii.

Tue May 15 00:00:00 EDT 2012

The nucleosome (histone-DNA complex) is the TLR9-specific immunostimulatory component of Plasmodium falciparum that activates DCs.

PloS one

Gowda NM,Wu X,Gowda DC

15-0862 was used in Flow cytometry/Cell sorting to provide a significant insight into the activation of DCs by malaria parasites.

Fri Oct 14 00:00:00 EDT 2011

Virulence of Toxoplasma gondii is associated with distinct dendritic cell responses and reduced numbers of activated CD8+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Tait ED,Jordan KA,Dupont CD,Harris TH,Gregg B,Wilson EH,Pepper M,Dzierszinski F,Roos DS,Hunter CA

15-0862 was used in Flow cytometry/Cell sorting to show the dendritic cell response to T.gondii alters levels of CD8+ T cells which ultimately determine the outcome of infection.

Sun Aug 01 00:00:00 EDT 2010

Pathogenic bacteria and dead cells are internalized by a unique subset of Peyer's patch dendritic cells that express lysozyme.

Gastroenterology

Lelouard H,Henri S,De Bovis B,Mugnier B,Chollat-Namy A,Malissen B,Méresse S,Gorvel JP

15-0862 was used in Flow cytometry/Cell sorting to investigate the expression of lysozyme in the Peyer's patches, showing that pathogenic bacteria and dead cells are internalised by a unique subset of dendritic cells expressing lysozyme.

Fri Jan 01 00:00:00 EST 2010

Identification of regulatory functions for 4-1BB and 4-1BBL in myelopoiesis and the development of dendritic cells.

Nature immunology

Lee SW,Park Y,So T,Kwon BS,Cheroutre H,Mittler RS,Croft M

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD86; early T cell costimulatory molecule-1; Early T-cell co-stimulatory molecule 1; Early T-cell costimulatory molecule 1; ETC-1; MGC34413; T-lymphocyte activation antigen CD86

Gene Aliases: B7; B7-2; B7.2; B70; Cd28l2; Cd86; CLS1; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2

UniProt ID: (Mouse) P42082

Entrez Gene ID: (Mouse) 12524

Molecular Function: immunoglobulin receptor superfamily