Invitrogen

CD8a Monoclonal Antibody (4SM16), eBioscience™

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Antibody Testing Data (1)
Published Figures (2)

CD8a Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD8a Antibody (14-0195-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded mouse spleen using 5 µg/mL Anti-Mouse CD8a Purified followed by Anti-Rat IgG Biotin, Streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.

CD8a Antibody in Immunohistochemistry (IHC)

CD8a Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

14-0195-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4SM16

Immunogen

Fragment of extracellular domain

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2637159

Product Specific Information

Description: The 4SM16 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

The 4SM16 monoclonal antibody is recommended for immunohistochemistry of formalin-fixed paraffin embedded (FFPE) or frozen mouse tissue and can be used for staining of mouse neural tissue. For additional information please contact our technical service (tech@eBioscience.com).

Applications Reported: This 4SM16 antibody has been reported for use in microscopy, immunohistochemical staining of frozen and formalin-fixed paraffin embedded (FFPE) tissue sections.

Applications Tested: This 4SM16 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This 4SM16 antibody has been tested by immunohistochemical staining of frozen mouse tissue and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Local and Abscopal Effect Observed with Liposomal Encapsulation of Intratumorally Injected Oncolytic Adenoviral Therapy.

Cancers

Dong T,Shah JR,Phung AT,Larson C,Sanchez AB,Aisagbonhi O,Blair SL,Oronsky B,Trogler WC,Reid T,Kummel AC

14-0195-82 was used in Immunohistochemistry-immunofluorescence to evaluate the in vivo therapeutic efficacy of oncolytic serotype 5 adenovirus TAV255 in CAR-deficient tumors.

Mon Jun 12 00:00:00 EDT 2023

Expansion of interferon inducible gene pool via USP18 inhibition promotes cancer cell pyroptosis.

Nature communications

Arimoto KI,Miyauchi S,Troutman TD,Zhang Y,Liu M,Stoner SA,Davis AG,Fan JB,Huang YJ,Yan M,Glass CK,Zhang DE

14-0195-82 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to show that USP18 suppression not only enhances expression of canonical IFN-stimulated genes (ISGs), but also activates the expression of a set of atypical ISGs and NF-κB target genes, including genes such as Polo like kinase 2 (PLK2), that induce cancer pyroptosis.

Tue Jan 17 00:00:00 EST 2023

Treatment of pancreatic cancer with irreversible electroporation and intratumoral CD40 antibody stimulates systemic immune responses that inhibit liver metastasis in an orthotopic model.

Journal for immunotherapy of cancer

Shankara Narayanan JS,Hayashi T,Erdem S,McArdle S,Tiriac H,Ray P,Pu M,Mikulski Z,Miller A,Messer K,Carson D,Schoenberger S,White RR

14-0195-82 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to generate meaningful therapeutic immune effects by combining IRE with local (intratumoral) delivery of a CD40 agonistic antibody (CD40Ab).

Sun Jan 01 00:00:00 EST 2023

Deficiency for SAMHD1 activates MDA5 in a cGAS/STING-dependent manner.

The Journal of experimental medicine

Schumann T,Ramon SC,Schubert N,Mayo MA,Hega M,Maser KI,Ada SR,Sydow L,Hajikazemi M,Badstübner M,Müller P,Ge Y,Shakeri F,Buness A,Rupf B,Lienenklaus S,Utess B,Muhandes L,Haase M,Rupp L,Schmitz M,Gramberg T,Manel N,Hartmann G,Zillinger T,Kato H,Bauer S,Gerbaulet A,Paeschke K,Roers A,Behrendt R

14-0195-82 was used in Immunohistochemistry to find that the chronic interferon response in SAMHD1-deficient mice was driven by the MDA5/MAVS pathway but required functional priming through the cGAS/STING pathway.

Mon Jan 02 00:00:00 EST 2023

Comparative analysis of the immune response to RFA and cryoablation in a colon cancer mouse model.

Scientific reports

Mauda-Havakuk M,Hawken NM,Owen JW,Mikhail AS,Saxena A,Karim B,Wakim PG,Pritchard WF,Karanian JW,Wood BJ

14-0195-82 was used in Immunohistochemistry to investigate and compare the immune responses to radiofrequency ablation (RFA) and cryoablation (CRA) in a colon cancer mouse model, revealing differences in the modulation of immune cells and cytokine profiles induced by these two ablation techniques.

Sat Oct 29 00:00:00 EDT 2022

Comparative analysis of the immune response to RFA and cryoablation in a colon cancer mouse model.

Scientific reports

Mauda-Havakuk M,Hawken NM,Owen JW,Mikhail AS,Saxena A,Karim B,Wakim PG,Pritchard WF,Karanian JW,Wood BJ

Sat Oct 29 00:00:00 EDT 2022

Host CLIC4 expression in the tumor microenvironment is essential for breast cancer metastatic competence.

PLoS genetics

Sanchez VC,Yang HH,Craig-Lucas A,Dubois W,Carofino BL,Lack J,Dwyer JE,Simpson RM,Cataisson C,Lee MP,Luo J,Hunter KW,Yuspa SH

14-0195-82 was used in Immunohistochemistry to show that CLIC4 expression in human breast cancers may serve as a prognostic biomarker; therapeutic targeting of CLIC4 could reduce primary tumor viability and host metastatic competence.

Wed Jun 01 00:00:00 EDT 2022

Clonally expanded CD8 T cells characterize amyotrophic lateral sclerosis-4.

Nature

Campisi L,Chizari S,Ho JSY,Gromova A,Arnold FJ,Mosca L,Mei X,Fstkchyan Y,Torre D,Beharry C,Garcia-Forn M,Jiménez-Alcázar M,Korobeynikov VA,Prazich J,Fayad ZA,Seldin MM,De Rubeis S,Bennett CL,Ostrow LW,Lunetta C,Squatrito M,Byun M,Shneider NA,Jiang N,La Spada AR,Marazzi I

14-0195-82 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to unravel disease mechanisms and as a potential biomarker of disease state.

Wed Jun 01 00:00:00 EDT 2022

Distinct Biomarker Profiles and TCR Sequence Diversity Characterize the Response to PD-L1 Blockade in a Mouse Melanoma Model.

Molecular cancer research : MCR

El Meskini R,Atkinson D,Kulaga A,Abdelmaksoud A,Gumprecht M,Pate N,Hayes S,Oberst M,Kaplan IM,Raber P,Van Dyke T,Sharan SK,Hollingsworth R,Day CP,Merlino G,Weaver Ohler Z

14-0195-82 was used in Immunohistochemistry to suggest that an anti-PD-L1-mediated melanoma regression response requires not only an expansion of the TCR repertoire that is unique to individual mice, but also tumor access to the appropriate TCRs.

Sun Aug 01 00:00:00 EDT 2021

CD95/Fas protects triple negative breast cancer from anti-tumor activity of NK cells.

iScience

Qadir AS,Guégan JP,Ginestier C,Chaibi A,Bessede A,Charafe-Jauffret E,Macario M,Lavoué V,Rouge TM,Law C,Vilker J,Wang H,Stroup E,Schipma MJ,Bridgeman B,Murmann AE,Ji Z,Legembre P,Peter ME

14-0195-82 was used in Immunohistochemistry to demonstrate that in addition to its tumor and metastasis promoting activities, CD95 expression by tumor cells can exert immune suppressive activities on NK cells, providing a new target for immune therapy.

Fri Nov 19 00:00:00 EST 2021

Retinoblastoma Inactivation Induces a Protumoral Microenvironment via Enhanced CCL2 Secretion.

Cancer research

Li F,Kitajima S,Kohno S,Yoshida A,Tange S,Sasaki S,Okada N,Nishimoto Y,Muranaka H,Nagatani N,Suzuki M,Masuda S,Thai TC,Nishiuchi T,Tanaka T,Barbie DA,Mukaida N,Takahashi C

14-0195 was used in Immunohistochemistry on paraffin embedded tissues to show that inactivation of the tumour suppressor retinoblastoma leads to a protumoral microenvironment via an increase in the secretion of the chemoattractant CCL2.

Thu Aug 01 00:00:00 EDT 2019

PD-1 Blockade Overcomes Adaptive Immune Resistance in Treatment with Anchored-GM-CSF Bladder Cancer Cells Vaccine.

Journal of Cancer

Zhang X,Shi X,Li J,Mo L,Hu Z,Gao J,Wu S,Long Z

Published figure using CD8a monoclonal antibody (Product # 14-0195-82) in Immunohistochemistry (PFA fixed)

Thu Oct 01 00:00:00 EDT 2020

PD-1 Blockade Overcomes Adaptive Immune Resistance in Treatment with Anchored-GM-CSF Bladder Cancer Cells Vaccine.

Journal of Cancer

Zhang X,Shi X,Li J,Mo L,Hu Z,Gao J,Wu S,Long Z

Published figure using CD8a monoclonal antibody (Product # 14-0195-82) in Immunohistochemistry (PFA fixed)

Thu Oct 01 00:00:00 EDT 2020

Ibuprofen supports macrophage differentiation, T cell recruitment, and tumor suppression in a model of postpartum breast cancer.

Journal for immunotherapy of cancer

Pennock ND,Martinson HA,Guo Q,Betts CB,Jindal S,Tsujikawa T,Coussens LM,Borges VF,Schedin P

14-0195 was used in Immunohistochemistry on paraffin embedded tissues to implicate beneficial prophylactic use of ibuprofen during the pro-tumorigenic window of mammary gland involution.

Mon Oct 01 00:00:00 EDT 2018

DNA methyltransferase inhibition upregulates MHC-I to potentiate cytotoxic T lymphocyte responses in breast cancer.

Nature communications

Luo N,Nixon MJ,Gonzalez-Ericsson PI,Sanchez V,Opalenik SR,Li H,Zahnow CA,Nickels ML,Liu F,Tantawy MN,Sanders ME,Manning HC,Balko JM

14-0195 was used in Immunohistochemistry-immunofluorescence to demonstrate up regulation of MHC-1 expression in human breast cancer in response to hypomethylating agent guadecitabine to aid anti-tumour immunity.

Tue Jan 16 00:00:00 EST 2018

DNA methyltransferase inhibition upregulates MHC-I to potentiate cytotoxic T lymphocyte responses in breast cancer.

Nature communications

Luo N,Nixon MJ,Gonzalez-Ericsson PI,Sanchez V,Opalenik SR,Li H,Zahnow CA,Nickels ML,Liu F,Tantawy MN,Sanders ME,Manning HC,Balko JM

Tue Jan 16 00:00:00 EST 2018

Bioinformatics

Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

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Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

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UniProt ID: (Mouse) P01731, (Mouse) P10300

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Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

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Function(s)

protein binding protein kinase binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8a Monoclonal Antibody (4SM16), eBioscience™

CD8a Antibody (14-0195-82) in IHC (P)

Product Details

14-0195-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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