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CD8a Antibody (740029T) in ICC/IF
Immunofluorescent analysis of CD8a was performed using CD8a transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with CD8a Recombinant Rat Monoclonal Antibody (4SM16) (Product # 740029T, 1:100) followed by Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (Product # A11006, 1:500). Panel a) shows representative images of cells that were stained for ... View More
Product Details
740029T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
4SM16
Immunogen
Fragment of extracellular domain
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant Rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen
Gene Aliases: BB154331; CD8; CD8A; CD8B; CD8B1; LEU2; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; LY3; Lyt-2; Lyt-3; Lyt2; LYT3; MAL; p32; P37
UniProt ID: (Human) P01732, (Human) P10966, (Mouse) P01731, (Mouse) P10300
Entrez Gene ID: (Human) 925, (Human) 926, (Mouse) 12525, (Mouse) 12526
T cell mediated immunity
immune response
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
antigen processing and presentation
T cell activation
cytotoxic T cell differentiation
regulation of immune response
positive regulation of calcium-mediated signaling
defense response to virus
adaptive immune response
regulation of defense response to virus by virus
immune system process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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