Invitrogen

CD8a Monoclonal Antibody (53-6.7), eFluor™ 615, eBioscience™

Product Image Gallery

CD8a Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry on frozen mouse spleen using 5 µg/mL Rat IgG2a Isotype Control eFluor® 615 (left) or 5 µg/mL Anti-Mouse CD8a eFluor® 615 (right). Nuclei are counterstained with DAPI.

CD8a Antibody in Immunocytochemistry (ICC/IF) — Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunocytochemistry

CD8a Antibody in Immunohistochemistry (IHC) — Fig. 6 Immunological responses after combined PTT-PDT-immunotherapy with CSPM@CpG. a Schematic illustration of CSPM@CpG and aPD-L1 combination therapy. b , c Representative flow cytometry data ( b ) and proportion of CTLs infiltration in tumors ( c ) (CD8 + Granzyme B + cells were defined as CTLs). d , e The production of IL-12 ( d ), TNF-alpha ( e ), and IFN-gamma ( f ) in serum of mice with different treatments. g Representative immunofluorescence images of tumor tissues from PBS and aPD-L1 + PDT + PTT group. 2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI)-labeled nuclei (blue); anti-CD8 + antibody-labeled T cells (green); anti-Granzyme B antibody-labeled T cells (pink). Scale bar: 100 mum. Data are presented as means +- standard deviation (s.d. ; n = 5). p values *<0.05, **<0.01, ***<0.001, and ****<0.0001.

CD8a Antibody in Western Blot (WB) — Figure 1. Reticuloendothelial tissues accumulate dark pigments in the absence of SLC48A1 . ( A ) Structure of the SLC48A1 gene (which encodes SLC48A1) indicating the CRISPR target site in exon 1. ( B ) Predicted topology of SLC48A1 protein; arrow indicates the site of the two basepair deletion resulting in frameshift mutation. ( C ) Immunoblot analysis of membrane lysates prepared from spleens and livers of mice. Membranes were probed with anti-SLC48A1 antibody and then incubated with HRP-conjugated anti-rabbit secondary antibody. Each lane represents one animal. ( D ) SLC48A1 immunohistochemistry analysis of paraffin-embedded tissue sections of mice. Tissue sections were probed with affinity-purified anti-SLC48A1 antibody and then incubated with HRP-conjugated anti-rabbit secondary antibody. Images shown are representative of at least three mice. ( E-F ) Spleen wet weights and whole blood hematocrit from WT and KO mice. Each dot represents one mouse; mice were age (6 weeks) and sex-matched. ( G ) Representative images of spleens, livers and bone marrows of age and sex-matched mice. *p<0.05. Figure 1-figure supplement 1. Genetic lesion in SLC48A1 , genotypic segregation and loss of SLC48A1 IHC by alkaline-phosphatase. ( A ) Chromatogram of genomic DNA sequencing from mice indicating two basepair deletion. ( B ) Mendelian distribution of P21 pups derived from SLC48A1 HET intercrosses. ( C ) SLC48A1 immunohistochemistry staining of secondary only controls on spleen, liver and b

CD8a Antibody in Immunoprecipitation (IP) — Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunoprecipitation

Product Details

42-0081-82

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 12 publications 12 publications
Immunohistochemistry (Frozen) (IHC (F))	5 µg/mL	-
Immunocytochemistry (ICC/IF)	Assay-Dependent	View 7 publications 7 publications
Immunoprecipitation (IP)	-	View 1 publication 1 publication
Functional Assay (FN)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 615, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	53-6.7
Conjugate	eFluor™ 615 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 561 Alexa Fluor 660 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 395 Brilliant UV 496 Brilliant UV 563 Brilliant UV 615 Brilliant UV 661 Brilliant UV 737 Brilliant UV 805 Brilliant Violet 421 Brilliant Violet 480 Brilliant Violet 650 Brilliant Violet 711 Brilliant Violet 786 eFluor 450 eFluor 506 eFluor 660 FITC
Excitation/Emission Max	595/614 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10805750

Product Specific Information

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Applications Reported: This 53-6.7 antibody has been reported for use in immunohistochemical staining of frozen tissue sections and immunocytochemistry.

Applications Tested: This 53-6.7 antibody has been tested by immunohistochemistry on frozen mouse spleen (IHC-F) at less than or equal to 5 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic potential of highly functional codon-optimized microutrophin for muscle-specific expression.

Scientific reports

Starikova AV,Skopenkova VV,Polikarpova AV,Reshetov DA,Vassilieva SG,Velyaev OA,Shmidt AA,Savchenko IM,Soldatov VO,Egorova TV,Bardina MV

42-0081-82 was used in Immunohistochemistry to investigate codon optimised gene therapies in alleviating side effects for future treatments.

Mon Jan 17 00:00:00 EST 2022

A Cu₉S₅ nanoparticle-based CpG delivery system for synergistic photothermal-, photodynamic- and immunotherapy.

Communications biology

Zhou L,Chen L,Hu X,Lu Y,Liu W,Sun Y,Yao T,Dong C,Shi S

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunohistochemistry

Fri Jul 03 00:00:00 EDT 2020

NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer.

Nature communications

Qin G,Wang X,Ye S,Li Y,Chen M,Wang S,Qin T,Zhang C,Li Y,Long Q,Hu H,Shi D,Li J,Zhang K,Zhai Q,Tang Y,Kang T,Lan P,Xie F,Lu J,Deng W

42-0081-82 was used in Immunocytochemistry to verify that triple-negative breast cancer (TNBC) has higher PD-L1 expression than other subtypes.

Fri Apr 03 00:00:00 EDT 2020

Stroma remodeling and reduced cell division define durable response to PD-1 blockade in melanoma.

Nature communications

Galvani E,Mundra PA,Valpione S,Garcia-Martinez P,Smith M,Greenall J,Thakur R,Helmink B,Andrews MC,Boon L,Chester C,Gremel G,Hogan K,Mandal A,Zeng K,Banyard A,Ashton G,Cook M,Lorigan P,Wargo JA,Dhomen N,Marais R

42-0081-82 was used in Immunohistochemistry to show the genetic and phenotypic changes induced by treatment with programmed cell death-1 (PD-1) blockade in a genetically engineered mouse model of melanoma driven by oncogenic BRAF.

Wed Feb 12 00:00:00 EST 2020

Hemozoin produced by mammals confers heme tolerance.

eLife

Pek RH,Yuan X,Rietzschel N,Zhang J,Jackson L,Nishibori E,Ribeiro A,Simmons W,Jagadeesh J,Sugimoto H,Alam MZ,Garrett L,Haldar M,Ralle M,Phillips JD,Bodine DM,Hamza I

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Western Blot

Tue Oct 01 00:00:00 EDT 2019

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

Wed Mar 28 00:00:00 EDT 2018

Type 2 Innate Lymphocytes Actuate Immunity Against Tumours and Limit Cancer Metastasis.

Scientific reports

Saranchova I,Han J,Zaman R,Arora H,Huang H,Fenninger F,Choi KB,Munro L,Pfeifer CG,Welch I,Takei F,Jefferies WA

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunohistochemistry

Tue Feb 13 00:00:00 EST 2018

MHC-I and PirB Upregulation in the Central and Peripheral Nervous System following Sciatic Nerve Injury.

PloS one

Bombeiro AL,Thomé R,Oliveira Nunes SL,Monteiro Moreira B,Verinaud L,Oliveira AL

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

Wed Jul 26 00:00:00 EDT 2017

The inflammatory phase of fracture healing is influenced by oestrogen status in mice.

European journal of medical research

Haffner-Luntzer M,Fischer V,Prystaz K,Liedert A,Ignatius A

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

Thu Jul 06 00:00:00 EDT 2017

Adeno-associated virus serotype rh.10 displays strong muscle tropism following intraperitoneal delivery.

Scientific reports

Ai J,Li J,Gessler DJ,Su Q,Wei Q,Li H,Gao G

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

Mon Jan 09 00:00:00 EST 2017

Postsynaptic SDC2 induces transsynaptic signaling via FGF22 for bidirectional synaptic formation.

Scientific reports

Hu HT,Umemori H,Hsueh YP

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunoprecipitation

Thu Sep 15 00:00:00 EDT 2016

Discovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33.

Scientific reports

Saranchova I,Han J,Huang H,Fenninger F,Choi KB,Munro L,Pfeifer C,Welch I,Wyatt AW,Fazli L,Gleave ME,Jefferies WA

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunohistochemistry

Tue Sep 13 00:00:00 EDT 2016

Metabolic maintenance of cell asymmetry following division in activated T lymphocytes.

Nature

Verbist KC,Guy CS,Milasta S,Liedmann S,Kamiński MM,Wang R,Green DR

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

Thu Apr 21 00:00:00 EDT 2016

MFG-E8 Is Critical for Embryonic Stem Cell-Mediated T Cell Immunomodulation.

Stem cell reports

Tan Y,AlKhamees B,Jia D,Li L,Couture JF,Figeys D,Jinushi M,Wang L

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

Tue Nov 10 00:00:00 EST 2015

T-bet Is Required for the Rapid Clearance of Attenuated Rabies Virus from Central Nervous System Tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lebrun A,Portocarrero C,Kean RB,Barkhouse DA,Faber M,Hooper DC

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

Sun Nov 01 00:00:00 EDT 2015

CD31 signals confer immune privilege to the vascular endothelium.

Proceedings of the National Academy of Sciences of the United States of America

Cheung K,Ma L,Wang G,Coe D,Ferro R,Falasca M,Buckley CD,Mauro C,Marelli-Berg FM

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunohistochemistry

Tue Oct 27 00:00:00 EDT 2015

Imaging Tolerance Induction in the Classic Medawar Neonatal Mouse Model: Active Roles of Multiple F1-Donor Cell Types.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Bascom RA,Tao KS,Tollenaar SL,West LJ

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunocytochemistry

Tue Sep 01 00:00:00 EDT 2015

Use of tilapia piscidin 3 (TP3) to protect against MRSA infection in mice with skin injuries.

Oncotarget

Huang HN,Chan YL,Hui CF,Wu JL,Wu CJ,Chen JY

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunohistochemistry

Sat May 30 00:00:00 EDT 2015

Adjuvant cationic liposomes presenting MPL and IL-12 induce cell death, suppress tumor growth, and alter the cellular phenotype of tumors in a murine model of breast cancer.

Molecular pharmaceutics

Meraz IM,Savage DJ,Segura-Ibarra V,Li J,Rhudy J,Gu J,Serda RE

42-0081 was used in Immunohistochemistry to show how combination liposomal therapy was able to induce similar reductions in tumor growth in both treated and distal tumors, suggesting a systemic immune response.

Mon Oct 06 00:00:00 EDT 2014

A missing PD-L1/PD-1 coinhibition regulates diabetes induction by preproinsulin-specific CD8 T-cells in an epitope-specific manner.

PloS one

Schuster C,Brosi H,Stifter K,Boehm BO,Schirmbeck R

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

Tue Apr 08 00:00:00 EDT 2014

In vivo CD8+ T cell dynamics in the liver of Plasmodium yoelii immunized and infected mice.

PloS one

Cabrera M,Pewe LL,Harty JT,Frevert U

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Immunofluorescence

Tue Mar 18 00:00:00 EDT 2014

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using CD8a monoclonal antibody (Product # 42-0081-82) in Western Blot

Fri Mar 01 00:00:00 EST 2013

Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2.

Investigative ophthalmology & visual science

Mochimaru H,Usui T,Yaguchi T,Nagahama Y,Hasegawa G,Usui Y,Shimmura S,Tsubota K,Amano S,Kawakami Y,Ishida S

Thu May 01 00:00:00 EDT 2008

Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes.

Circulation

Yang Z,Day YJ,Toufektsian MC,Xu Y,Ramos SI,Marshall MA,French BA,Linden J

Tue Nov 07 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

UniProt ID: (Mouse) P01731, (Mouse) P10300

Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

Molecular Function: defense/immunity protein immunoglobulin receptor superfamily