|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Mouse CD8 β|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1 ug/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17843 targets CD8b/LYT3 in FACS applications and shows reactivity with Mouse samples.
The MA5-17843 immunogen is mouse CD8 ß.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Maternal immune stimulation during pregnancy shapes the immunological phenotype of offspring.
MA5-17843 was used in flow cytometry to use poly(I:C)-injected dams to study the effects of inflammation on fetal development.
|Mandal M,Donnelly R,Elkabes S,Zhang P,Davini D,David BT,Ponzio NM||Brain, behavior, and immunity (33:33)||2013|
Anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement and improve peptide-MHCI tetramer staining.
MA5-17843 was used in flow cytometry to test if anti-CD8 Abs induce effector function.
|Clement M,Ladell K,Ekeruche-Makinde J,Miles JJ,Edwards ES,Dolton G,Williams T,Schauenburg AJ,Cole DK,Lauder SN,Gallimore AM,Godkin AJ,Burrows SR,Price DA,Sewell AK,Wooldridge L||Journal of immunology (Baltimore, Md. : 1950) (187:654)||2011|
|Not Applicable||Not Cited||
Defective proximal TCR signaling inhibits CD8+ tumor-infiltrating lymphocyte lytic function.
MA5-17843 was used to elucidate the lack of cytolysis of CD8+ tumor-infiltrating lymphocytes
|Koneru M,Schaer D,Monu N,Ayala A,Frey AB||Journal of immunology (Baltimore, Md. : 1950) (174:1830)||2005|
The point mutation of tyrosine 759 of the IL-6 family cytokine receptor gp130 synergizes with HTLV-1 pX in promoting rheumatoid arthritis-like arthritis.
MA5-17843 was used in flow cytometry to characterize a new mouse model of rheumatoid arthritis.
|Ishihara K,Sawa S,Ikushima H,Hirota S,Atsumi T,Kamimura D,Park SJ,Murakami M,Kitamura Y,Iwakura Y,Hirano T||International immunology (16:455)||2004|
High avidity antigen-specific CTL identified by CD8-independent tetramer staining.
MA5-17843 was used in flow cytometry to test if tetramers distinguish between high and low avidity T cell clones.
|Choi EM,Chen JL,Wooldridge L,Salio M,Lissina A,Lissin N,Hermans IF,Silk JD,Mirza F,Palmowski MJ,Dunbar PR,Jakobsen BK,Sewell AK,Cerundolo V||Journal of immunology (Baltimore, Md. : 1950) (171:5116)||2003|
Endogenous inhibition of antimycobacterial immunity by IL-10 varies between mycobacterial species.
MA5-17843 was used in flow cytometry to elucidate the role of IL-10 in mycobacterial infections.
|Roach DR,Martin E,Bean AG,Rennick DM,Briscoe H,Britton WJ||Scandinavian journal of immunology (54:163)||2001|
CD8+ tumor-infiltrating lymphocytes are primed for Fas-mediated activation-induced cell death but are not apoptotic in situ.
MA5-17843 was used in flow cytometry to characterize murine tumor-infiltrating lymphocytes.
|Radoja S,Saio M,Frey AB||Journal of immunology (Baltimore, Md. : 1950) (166:6074)||2001|
Characterization of peripheral regulatory CD4+ T cells that prevent diabetes onset in nonobese diabetic mice.
MA5-17843 was used in flow cytometry to determine the phenotype and function of T cells that prevent diabetes.
|Lepault F,Gagnerault MC||Journal of immunology (Baltimore, Md. : 1950) (164:240)||2000|
Lymphoid hyperplasia, autoimmunity, and compromised intestinal intraepithelial lymphocyte development in colitis-free gnotobiotic IL-2-deficient mice.
MA5-17843 was used in flow cytometry to determine if the microbiota contributes to autoimmunity in IL-2 deficient mice.
|Contractor NV,Bassiri H,Reya T,Park AY,Baumgart DC,Wasik MA,Emerson SG,Carding SR||Journal of immunology (Baltimore, Md. : 1950) (160:385)||1998|
Targets of p56(lck) activity in immature thymoblasts: stimulation of the Ras/Raf/MAPK pathway.
MA5-17843 was used in flow cytometry to demonstrate that the Ras/Raf/MAPK pathway is constitutively activated in lck-transformed immature thymoblasts.
|Lin K,Abraham KM||International immunology (9:291)||1997|
|Not Applicable||Not Cited||
Inactivation of tumor-specific CD8¿ CTLs by tumor-infiltrating tolerogenic dendritic cells.
MA5-17843 was used in flow cytometry to elucidate the role of dendritic cells in cancer immunosurveillance failure
|Harimoto H,Shimizu M,Nakagawa Y,Nakatsuka K,Wakabayashi A,Sakamoto C,Takahashi H||Immunology and cell biology (91:545)||2013|
|Not Applicable||Not Cited||
Thymic alterations in mice deficient for the SNARE protein VAMP8/endobrevin.
MA5-17843 was used in flow cytometry to elucidate the role of VAMP8 in the thymus
|Kanwar N,Fayyazi A,Backofen B,Nitsche M,Dressel R,von Mollard GF||Cell and tissue research (334:227)||2008|