|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Mouse CD8 β|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1 ug/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17848 targets CD8b/LYT3 in FACS applications and shows reactivity with Mouse samples.
The MA5-17848 immunogen is mouse CD8 ß.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Inactivation of tumor-specific CD8¿ CTLs by tumor-infiltrating tolerogenic dendritic cells.
MA5-17848 was used in flow cytometry to elucidate the role of dendritic cells in cancer immunosurveillance failure
|Harimoto H,Shimizu M,Nakagawa Y,Nakatsuka K,Wakabayashi A,Sakamoto C,Takahashi H||Immunology and cell biology (91:545)||2013|
Maternal immune stimulation during pregnancy affects adaptive immunity in offspring to promote development of TH17 cells.
MA5-17848 was used in flow cytometry to examine how maternal immune stimulation affects the adaptive immune system and pathobiology of the offspring
|Mandal M,Marzouk AC,Donnelly R,Ponzio NM||Brain, behavior, and immunity (25:863)||2011|
Lymphoid precursors in intestinal cryptopatches express CCR6 and undergo dysregulated development in the absence of CCR6.
MA5-17848 was used in flow cytometry to study CCR6 expression by T lymphocyte precursors undergoing extrathymic differentiation in intestinal cryptopatches.
|Lügering A,Kucharzik T,Soler D,Picarella D,Hudson JT,Williams IR||Journal of immunology (Baltimore, Md. : 1950) (171:2208)||2003|
Optimal colocalization of TCR and CD8 as a novel mechanism for the control of functional avidity.
MA5-17848 was used in flow cytometry to investigate the control of functional avidity in response to viral infection.
|Cawthon AG,Alexander-Miller MA||Journal of immunology (Baltimore, Md. : 1950) (169:3492)||2002|
Cellular and molecular characterization of the scurfy mouse mutant.
MA5-17848 was used in flow cytometry to further characterize mice hemizygous for scurfy.
|Clark LB,Appleby MW,Brunkow ME,Wilkinson JE,Ziegler SF,Ramsdell F||Journal of immunology (Baltimore, Md. : 1950) (162:2546)||1999|