|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Mouse CD8 β|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1 ug/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17849 targets CD8b/LYT3 in FACS applications and shows reactivity with Mouse samples.
The MA5-17849 immunogen is mouse CD8 ß.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Thymic alterations in mice deficient for the SNARE protein VAMP8/endobrevin.
MA5-17849 was used in flow cytometry to elucidate the role of VAMP8 in the thymus
|Kanwar N,Fayyazi A,Backofen B,Nitsche M,Dressel R,von Mollard GF||Cell and tissue research (334:227)||2008|
Immunoglobulin and cytokine production from spleen lymphocytes is modulated in C57BL/6J mice by dietary cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid.
MA5-17849 was used in flow cytometry to study the effect of dietary cis-9, trans- and trans-0, cis-2 conjugated linoleic acid on immunoglobulin and cytokine production from spleen lymphocytes in C57BL/6J mice.
|Yamasaki M,Chujo H,Hirao A,Koyanagi N,Okamoto T,Tojo N,Oishi A,Iwata T,Yamauchi-Sato Y,Yamamoto T,Tsutsumi K,Tachibana H,Yamada K||The Journal of nutrition (133:784)||2003|
Characterization of peripheral regulatory CD4+ T cells that prevent diabetes onset in nonobese diabetic mice.
MA5-17849 was used in flow cytometry to determine the phenotype and function of T cells that prevent diabetes.
|Lepault F,Gagnerault MC||Journal of immunology (Baltimore, Md. : 1950) (164:240)||2000|
Impaired immune responses and B-cell proliferation in mice lacking the Id3 gene.
MA5-17849 was used in flow cytometry to investigate the role of Id3 in tissue and embryo development.
|Pan L,Sato S,Frederick JP,Sun XH,Zhuang Y||Molecular and cellular biology (19:5969)||1999|