Invitrogen

CD9 Monoclonal Antibody (MM2/57)

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CD9 Antibody in Western Blot (WB) — Characterization of exosome fractions concentrated from serum. ( a ) Dynamic light scattering measurements. Size and zeta potential distribution (10 measurements, each) for samples concentrated from pregnant women without (CG) and with diabetes (GDM). ( b ) Detection of exosome markers CD9 and CD63 by Western blot in serum exosome fractions concentrated from CG and GDM. ( c ) Principal component analysis plot of the exosome differentially abundant proteins. A distinct proteome can be discerned in GDM compared with CG group.

CD9 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Fig. 6 EXi-RVFV Depletion Eliminates Interferon-B Induction in Naive Recipient Cells. A The sucrose density fractions containing EXi-RVFV, the most bottom fraction containing virus (density of 1.25 g ml -1 ), and the working stock of MP12 strain (1.97 x 10 7 pfu/ml), were analyzed by western blot for the exosome marker CD9. B EXi-RVFV samples were subjected to immunoprecipitation using magnetic Dynabeads coated with human anti-CD9 antibody. The efficacy of pull-down was analyzed by anti-CD9 immunoblot of the starting material (Input EXi-RVFV), the immunoprecipitated EXi-RVFV, and the EXi-depleted supernatant that was left behind (DeltaEXi). Three biological repeats were performed. A typical western result is shown. C Naive U937 cells were either left untreated, or were subjected to one of the following treatments: i) Poly(I:C); ii) EXu; iii) EXi-RVFV; iv) DeltaEXi. The IFN-B expression levels in treated cells were then quantified by RT-qPCR analysis. For each treatment condition, mean value +- SEM from three biological replicates is shown (***P <= 0.005). D Anti-IFN-B immunoblot analysis was performed to compare IFN-B induction in naive U937 cells treated with either EXi-RVFV or samples depleted of EXi-RVFV by anti-CD9 IP pulldown (DeltaEXi). The intracellular IFN-B expression was analyzed at 24 h post treatment. Untreated cells, cells treated with EXu, or cells infected with either the MP12 strain or the DeltaNSs mutant strain of RVFV, were also analyzed side by side. For ea

Product Details

MA1-80307

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:500-1:1,000

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1/100 - 1/200

View 1 publication 1 publication

Immunoprecipitation (IP)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Cat, Human, Mink, Pig, Rabbit, Rhesus monkey

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

MM2/57

Immunogen

Human platelet membrane.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_929680

Product Specific Information

Predicted to react with mustelid based on sequence homology.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD9 antibody, clone MM2/57 recognizes human leukocyte antigen MIC3 also known as MRP-1 or CD9.

Target Information

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosome Proteomics Reveals the Deregulation of Coagulation, Complement and Lipid Metabolism Proteins in Gestational Diabetes Mellitus.

Molecules (Basel, Switzerland)

Bernea EG,Suica VI,Uyy E,Cerveanu-Hogas A,Boteanu RM,Ivan L,Ceausu I,Mihai DA,Ionescu-Tîrgoviște C,Antohe F

Published figure using CD9 monoclonal antibody (Product # MA1-80307) in Western Blot

Fri Aug 26 00:00:00 EDT 2022

A Monoclonal Anti-HMGB1 Antibody Attenuates Neurodegeneration in an Experimental Animal Model of Glaucoma.

International journal of molecular sciences

Tonner H,Hunn S,Auler N,Schmelter C,Beutgen VM,von Pein HD,Pfeiffer N,Grus FH

MA1-80307 was used in Immunohistochemistry to investigate the effects of the intravitreal injection of an anti-HMGB1 monoclonal antibody (anti-HMGB1 Ab) in an experimental animal model of glaucoma.

Thu Apr 07 00:00:00 EDT 2022

Exosomes originating from infection with the cytoplasmic single-stranded RNA virus Rift Valley fever virus (RVFV) protect recipient cells by inducing RIG-I mediated IFN-B response that leads to activation of autophagy.

Cell & bioscience

Alem F,Olanrewaju AA,Omole S,Hobbs HE,Ahsan N,Matulis G,Brantner CA,Zhou W,Petricoin EF,Liotta LA,Caputi M,Bavari S,Wu Y,Kashanchi F,Hakami RM

MA1-80307 was used in Immunoprecipitation to investigate cellular effects of exosomes released upon infection of Rift Valley fever virus.

Sat Dec 25 00:00:00 EST 2021

Cell & bioscience

Alem F,Olanrewaju AA,Omole S,Hobbs HE,Ahsan N,Matulis G,Brantner CA,Zhou W,Petricoin EF,Liotta LA,Caputi M,Bavari S,Wu Y,Kashanchi F,Hakami RM

MA1-80307 was used in Immunoprecipitation to investigate cellular effects of exosomes released upon infection of Rift Valley fever virus.

Sat Dec 25 00:00:00 EST 2021

Cell & bioscience

Alem F,Olanrewaju AA,Omole S,Hobbs HE,Ahsan N,Matulis G,Brantner CA,Zhou W,Petricoin EF,Liotta LA,Caputi M,Bavari S,Wu Y,Kashanchi F,Hakami RM

MA1-80307 was used in Immunoprecipitation to investigate cellular effects of exosomes released upon infection of Rift Valley fever virus.

Sat Dec 25 00:00:00 EST 2021

Size-Exclusion Chromatography Separation Reveals That Vesicular and Non-Vesicular Small RNA Profiles Differ in Cell Free Urine.

International journal of molecular sciences

Karttunen J,Stewart SE,Kalmar L,Grant AJ,Karet Frankl FE,Williams TL

Published figure using CD9 monoclonal antibody (Product # MA1-80307) in Western Blot

Wed May 05 00:00:00 EDT 2021

Biosynthesized Multivalent Lacritin Peptides Stimulate Exosome Production in Human Corneal Epithelium.

International journal of molecular sciences

Lee C,Edman MC,Laurie GW,Hamm-Alvarez SF,MacKay JA

Published figure using CD9 monoclonal antibody (Product # MA1-80307) in Western Blot

Wed Aug 26 00:00:00 EDT 2020

Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk.

Cell communication and signaling : CCS

Es-Haghi M,Godakumara K,Häling A,Lättekivi F,Lavrits A,Viil J,Andronowska A,Nafee T,James V,Jaakma Ü,Salumets A,Fazeli A

Published figure using CD9 monoclonal antibody (Product # MA1-80307) in Western Blot

Thu Nov 14 00:00:00 EST 2019

CD151-α3β1 integrin complexes are prognostic markers of glioblastoma and cooperate with EGFR to drive tumor cell motility and invasion.

Oncotarget

Zhou P,Erfani S,Liu Z,Jia C,Chen Y,Xu B,Deng X,Alfáro JE,Chen L,Napier D,Lu M,Huang JA,Liu C,Thibault O,Segal R,Zhou BP,Kyprianou N,Horbinski C,Yang XH

MA1-80307 was used in flow cytometry to elucidate the role of CD151-alpha3beta1 integrin complexes in its cooperation with EGFR to drive tumor cell motility and invasion and as prognositic markers of glioblastoma

Tue Oct 06 00:00:00 EDT 2015

Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.

PloS one

Jin Y,Tachibana I,Takeda Y,He P,Kang S,Suzuki M,Kuhara H,Tetsumoto S,Tsujino K,Minami T,Iwasaki T,Nakanishi K,Kohmo S,Hirata H,Takahashi R,Inoue K,Nagatomo I,Kida H,Kijima T,Ito M,Saya H,Kumanogoh A

MA1-80307 was used in western blot to identify agents that increase the levels of tetraspanins in macrophages.

Wed Jul 02 00:00:00 EDT 2014

Native type IV collagen induces cell migration through a CD9 and DDR1-dependent pathway in MDA-MB-231 breast cancer cells.

European journal of cell biology

Castro-Sanchez L,Soto-Guzman A,Navarro-Tito N,Martinez-Orozco R,Salazar EP

MA1-80307 was used in western blot to elucidate the contribution of DDR1 and type IV collagen to regulation of CD9-cell surface levels and migration of breast cancer cells

Mon Nov 01 00:00:00 EDT 2010

Cell surface tetraspanin CD9 mediates chemoresistance in small cell lung cancer.

Cancer research

Kohmo S,Kijima T,Otani Y,Mori M,Minami T,Takahashi R,Nagatomo I,Takeda Y,Kida H,Goya S,Yoshida M,Kumagai T,Tachibana I,Yokota S,Kawase I

MA1-80307 was used in flow cytometry and western blot to investigate the role of CD9 in the cell adhesion-mediated drug resistance mechanism of small cell lung cancer

Fri Oct 15 00:00:00 EDT 2010

Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis.

Molecular biology of the cell

Lafleur MA,Xu D,Hemler ME

MA1-80307 was used in immunocytochemistry and western blot to investigate the role of MT1-MMP-tetraspanin complexes in cancer cell invasion and metastasis

Wed Apr 01 00:00:00 EDT 2009

Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice.

The Journal of biological chemistry

Takeda Y,He P,Tachibana I,Zhou B,Miyado K,Kaneko H,Suzuki M,Minami S,Iwasaki T,Goya S,Kijima T,Kumagai T,Yoshida M,Osaki T,Komori T,Mekada E,Kawase I

MA1-80307 was used in western blot to examine the roles of CD9 and CD81 in cell motility and protease production of smoke-treated macrophages

Fri Sep 19 00:00:00 EDT 2008

CD151 accelerates breast cancer by regulating alpha 6 integrin function, signaling, and molecular organization.

Cancer research

Yang XH,Richardson AL,Torres-Arzayus MI,Zhou P,Sharma C,Kazarov AR,Andzelm MM,Strominger JL,Brown M,Hemler ME

MA1-80307 was used in western blot to determine the contribution of CD151 on breast cancer

Thu May 01 00:00:00 EDT 2008

Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells.

Cancer research

Saito Y,Tachibana I,Takeda Y,Yamane H,He P,Suzuki M,Minami S,Kijima T,Yoshida M,Kumagai T,Osaki T,Kawase I

MA1-80307 was used in flow cytometry, immunoprecipitation, and western blot to investigate the role of CD9 in small cell lung cancer cells

Sun Oct 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: 5H9; 5H9 antigen; antigen CD9; BA-2/p24 antigen; BA2; CD9; CD9 antigen; CD9 antigen (p24); Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; mamu-MICA/B; MHC class I polypeptide-related sequence 3; MHC class I-related chain; motility related protein-1; Motility-related protein; MRP-1; p24; sCD 9; sCD9; soluble CD 9; soluble CD9; Tetraspanin-29; Tetraspanin29; Tspan-29

Gene Aliases: BTCC-1; CD9; DRAP-27; GIG2; Mamu-MIC1; MIC3; MRP-1; TSPAN-29; TSPAN29

UniProt ID: (Pig) Q8WMQ3, (Human) P21926, (Bovine) P30932, (Cat) P40239

Entrez Gene ID: (Pig) 397067, (Rhesus monkey) 712262, (Rabbit) 100345844, (Human) 928, (Bovine) 280746, (Horse) 100059512, (Cat) 493874, (Dog) 611695, (Rhesus monkey) 100141395

Molecular Function: major histocompatibility complex protein