Invitrogen

CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), APC-eFluor™ 780, eBioscience™

Product Image Gallery

CD90.1 (Thy-1.1) Antibody in Flow Cytometry (Flow) — Staining of rat splenocytes with Anti-Rat CD3 FITC (Product # 11-0030-82) and 0.03 µg of Mouse IgG2a kappa Isotype Control APC-eFluor® 780 (Product # 47-4724) (left) or 0.03 µg of Anti-Mouse/Rat CD90-1 (Thy-1-1) APC-eFluor® 780 (right). Total viable cells were used for analysis.

Product Details

47-0900-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.06 µg/test	View 33 publications 33 publications

Product Specifications
Species Reactivity	Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	HIS51
Conjugate	APC-eFluor™ 780 APC Biotin eFluor 450 FITC PE PE-Cyanine5 PE-Cyanine7 PerCP-Cyanine5.5 Super Bright 436 Super Bright 600 Super Bright 645 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_1272252

Product Specific Information

Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.

Applications Reported: This HIS51 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIS51 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Sustained release of exosomes loaded into polydopamine-modified chitin conduits promotes peripheral nerve regeneration in rats.

Neural regeneration research

Li C,Liu SY,Zhang M,Pi W,Wang B,Li QC,Lu CF,Zhang PX

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2022

Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium.

Frontiers in bioengineering and biotechnology

Guo Q,Chang Y,Li J,Zhou C,Huang R,Yang X,Liu G,Liang X

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Sat Jul 16 00:00:00 EDT 2022

Binary outcomes of enhancer activity underlie stable random monoallelic expression.

eLife

Kissiov DU,Ethell A,Chen S,Wolf NK,Zhang C,Dang SM,Jo Y,Madsen KN,Paranjpe I,Lee AY,Chim B,Muljo SA,Raulet DH

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Thu May 26 00:00:00 EDT 2022

Propofol suppresses adipose-derived stem cell progression via PI3K/AKT-Wnt signaling pathway.

BMC anesthesiology

Yin G,Wang J,Zhong Y,Wu W

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2022

Distinct skeletal stem cell types orchestrate long bone skeletogenesis.

eLife

Ambrosi TH,Sinha R,Steininger HM,Hoover MY,Murphy MP,Koepke LS,Wang Y,Lu WJ,Morri M,Neff NF,Weissman IL,Longaker MT,Chan CK

47-0900-82 was used in Flow Cytometry to address previously unappreciated shortcomings of SSC research.

Mon Jul 19 00:00:00 EDT 2021

Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway.

Stem cell research & therapy

Ebrahim N,Dessouky AA,Mostafa O,Hassouna A,Yousef MM,Seleem Y,El Gebaly EAEAM,Allam MM,Farid AS,Saffaf BA,Sabry D,Nawar A,Shoulah AA,Khalil AH,Abdalla SF,El-Sherbiny M,Elsherbiny NM,Salim RF

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Tue Jul 13 00:00:00 EDT 2021

TNF-α-induced alterations in stromal progenitors enhance leukemic stem cell growth via CXCR2 signaling.

Cell reports

Agarwal P,Li H,Choi K,Hueneman K,He J,Welner RS,Starczynowski DT,Bhatia R

47-0900-82 was used in Flow Cytometry to find that TNF-α-mediated alterations in CML BM stromal niches enhance support of LSC maintenance and growth via CXCL1-CXCR2 signaling and that CXCR2 inhibition effectively depletes CML LSCs.

Tue Jul 13 00:00:00 EDT 2021

Macrophage polarization by MSC-derived CXCL12 determines tumor growth.

Cellular & molecular biology letters

Babazadeh S,Nassiri SM,Siavashi V,Sahlabadi M,Hajinasrollah M,Zamani-Ahmadmahmudi M

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Sat Jun 26 00:00:00 EDT 2021

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

Niche-Selective Inhibition of Pathogenic Th17 Cells by Targeting Metabolic Redundancy.

Cell

Wu L,Hollinshead KER,Hao Y,Au C,Kroehling L,Ng C,Lin WY,Li D,Silva HM,Shin J,Lafaille JJ,Possemato R,Pacold ME,Papagiannakopoulos T,Kimmelman AC,Satija R,Littman DR

47-0900-82 was used in Flow Cytometry to suggest that inhibiting glycolysis by targeting Gpi1 could be an effective therapeutic strategy with minimum toxicity for Th17-mediated autoimmune diseases, and, more generally, that metabolic redundancies can be exploited for selective targeting of disease processes.

Thu Aug 06 00:00:00 EDT 2020

Effects of bone marrow‑derived mesenchymal stem cell transplantation on chronic obstructive pulmonary disease/obstructive sleep apnea overlap syndrome in rats.

Molecular medicine reports

Chen M,Huang Z,Bi H,Pan X,He J,He L,He X,Du J,Zhou K,Wang L,Wang Q,Guo X,Jin Z

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Protective mechanism of artemisinin on rat bone marrow-derived mesenchymal stem cells against apoptosis induced by hydrogen peroxide via activation of c-Raf-Erk1/2-p90^rsk-CREB pathway.

Stem cell research & therapy

Fang J,Zhao X,Li S,Xing X,Wang H,Lazarovici P,Zheng W

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Sat Oct 26 00:00:00 EDT 2019

Co-culture of the bone and bone marrow: a novel way to obtain mesenchymal stem cells with enhanced osteogenic ability for fracture healing in SD rats.

Journal of orthopaedic surgery and research

Zhu C,Sha M,Jiang H,Lin J,Lin W,Li W,Chen X,Huang G,Ding Z

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Tue Sep 03 00:00:00 EDT 2019

Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model.

Medical science monitor : international medical journal of experimental and clinical research

Chi L,Huang Y,Mao Y,Wu K,Zhang L,Nan G

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Mon Jun 11 00:00:00 EDT 2018

Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43.

Experimental and therapeutic medicine

Li Z,Zhang S,Cao L,Li W,Ye YC,Shi ZX,Wang ZR,Sun LX,Wang JW,Jia LT,Wang W

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Yi Guan Jian decoction may enhance hepatic differentiation of bone marrow‑derived mesenchymal stem cells via SDF‑1 in vitro.

Molecular medicine reports

Fu LL,Pang BY,Zhu Y,Wang L,Leng AJ,Chen HL

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Tissue-engineered sling with adipose-derived stem cells under static mechanical strain.

Experimental and therapeutic medicine

Wang Y,Wang W,Wang X,Wang Y,Wang J,Fu Q,Shi G

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Mutations in 5-methylcytosine oxidase TET2 and RhoA cooperatively disrupt T cell homeostasis.

The Journal of clinical investigation

Zang S,Li J,Yang H,Zeng H,Han W,Zhang J,Lee M,Moczygemba M,Isgandarova S,Yang Y,Zhou Y,Rao A,You MJ,Sun D,Huang Y

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

CD73-derived adenosine and tenascin-C control cytokine production by epicardium-derived cells formed after myocardial infarction.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hesse J,Leberling S,Boden E,Friebe D,Schmidt T,Ding Z,Dieterich P,Deussen A,Roderigo C,Rose CR,Floss DM,Scheller J,Schrader J

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo.

International journal of molecular medicine

Wang D,Ding X,Xue W,Zheng J,Tian X,Li Y,Wang X,Song H,Liu H,Luo X

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Identification of a CD133-CD55- population functions as a fetal common skeletal progenitor.

Scientific reports

Weng L,Hu X,Kumar B,Garcia M,Todorov I,Jung X,Marcucci G,Forman SJ,Chen CC

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Thu Dec 08 00:00:00 EST 2016

Treatment of radiation-induced acute intestinal injury with bone marrow-derived mesenchymal stem cells.

Experimental and therapeutic medicine

Zheng K,Wu W,Yang S,Huang L,Chen J,Gong C,Fu Z,Lin R,Tan J

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Fibroblast Growth Factor-10 (FGF-10) Mobilizes Lung-resident Mesenchymal Stem Cells and Protects Against Acute Lung Injury.

Scientific reports

Tong L,Zhou J,Rong L,Seeley EJ,Pan J,Zhu X,Liu J,Wang Q,Tang X,Qu J,Bai C,Song Y

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Fri Feb 12 00:00:00 EST 2016

Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro.

Molecular medicine reports

Hu Y,Zhang Y,Tian K,Xun C,Wang S,Lv D

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Mesenchymal Cancer Cell-Stroma Crosstalk Promotes Niche Activation, Epithelial Reversion, and Metastatic Colonization.

Cell reports

Del Pozo Martin Y,Park D,Ramachandran A,Ombrato L,Calvo F,Chakravarty P,Spencer-Dene B,Derzsi S,Hill CS,Sahai E,Malanchi I

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Tue Dec 22 00:00:00 EST 2015

Isolation of adipose and bone marrow mesenchymal stem cells using CD29 and CD90 modifies their capacity for osteogenic and adipogenic differentiation.

Journal of tissue engineering

Davies OG,Cooper PR,Shelton RM,Smith AJ,Scheven BA

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Fri Sep 18 00:00:00 EDT 2015

Diverse roles for T-bet in the effector responses required for resistance to infection.

Journal of immunology (Baltimore, Md. : 1950)

Harms Pritchard G,Hall AO,Christian DA,Wagage S,Fang Q,Muallem G,John B,Glatman Zaretsky A,Dunn WG,Perrigoue J,Reiner SL,Hunter CA

47-0900 was used in Flow cytometry/Cell sorting to highlight T-bet-independent pathways to IFN-γ production and reveal a novel role for this transcription factor in coordinating the T cell responses necessary to control infection in peripheral tissues.

Sun Feb 01 00:00:00 EST 2015

Developmental heterogeneity of cardiac fibroblasts does not predict pathological proliferation and activation.

Circulation research

Ali SR,Ranjbarvaziri S,Talkhabi M,Zhao P,Subat A,Hojjat A,Kamran P,Müller AM,Volz KS,Tang Z,Red-Horse K,Ardehali R

47-0900 was used in Flow cytometry/Cell sorting to study the developmental origins of cardiac fibroblasts and characterise their corresponding phenotypes, to aid in determining the proliferation rates of each developmental subset.

Fri Sep 12 00:00:00 EDT 2014

Injury-dependent retention of intraportally administered mesenchymal stromal cells following partial hepatectomy of steatotic liver does not lead to improved liver recovery.

PloS one

Boeykens N,Ponsaerts P,Van der Linden A,Berneman Z,Ysebaert D,De Greef K

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Thu Feb 13 00:00:00 EST 2014

Inefficient TLR4/MD-2 heterotetramerization by monophosphoryl lipid A.

PloS one

Casella CR,Mitchell TC

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Acid ceramidase maintains the chondrogenic phenotype of expanded primary chondrocytes and improves the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells.

PloS one

Simonaro CM,Sachot S,Ge Y,He X,Deangelis VA,Eliyahu E,Leong DJ,Sun HB,Mason JB,Haskins ME,Richardson DW,Schuchman EH

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Tracking epitope-specific T cells.

Nature protocols

Moon JJ,Chu HH,Hataye J,Pagán AJ,Pepper M,McLachlan JB,Zell T,Jenkins MK

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 47-0900-82) in Flow Cytometry

Tue Jun 30 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; thy-1 glycoprotein; Thy-1 membrane glycoprotein; Thy-1 protein; thymus cell antigen 1, theta; Thymus cell surface antigen

Gene Aliases: CD7; CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

UniProt ID: (Mouse) P01831, (Rat) P01830

Entrez Gene ID: (Mouse) 21838, (Rat) 24832

Molecular Function: cell adhesion molecule