Invitrogen

CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), APC-eFluor 780, eBioscience™

20 Published Figures

16 References

Datasheet

Datasheet

Product Image Gallery

CD90.1 (Thy-1.1) Antibody — Figure 1 Identification of ADMSCs. ( A ) Flow cytometry detection of surface antigens on ADMSCs: after induction, the CD29+, CD40+, and CD90+ cells were the highest populations of stem cells. ( B ) Representative images of osteogenic differentiation of ADMSCs as detected by Alizarin Red S method; osteogenic ADMSCs were stained red. ( C ) representative images of adipogenic differentiation of ADMSCs as detected by Oil Red O method; adipogenic ADMSCs were stained red. Magnification: 400x.

CD90.1 (Thy-1.1) Antibody in Flow Cytometry (Flow) — Staining of rat splenocytes with Anti-Rat CD3 FITC (Product # 11-0030-82) and 0.03 µg of Mouse IgG2a kappa Isotype Control APC-eFluor® 780 (Product # 47-4724) (left) or 0.03 µg of Anti-Mouse/Rat CD90-1 (Thy-1-1) APC-eFluor® 780 (right). Total viable cells were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.06 µg/test

Published Applications

Flow Cytometry (Flow)

See 16 publications below

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse

Host / Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

HIS51

Conjugate

APC-eFluor® 780

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_1272252

Product Specific Information

Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.

Applications Reported: This HIS51 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIS51 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 glycoprotein; Thy-1 membrane glycoprotein; Thy-1 protein; Thy-1 T-cell antigen; THY1; thymus cell antigen 1, theta; Thymus cell surface antigen

Gene Aliases: CD7; CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

UniProt ID: (Mouse) P01831, (Rat) P01830

Entrez Gene ID: (Mouse) 21838, (Rat) 24832

Function(s)