Invitrogen

CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), PE, eBioscience™

Product Image Gallery

CD90 (Thy-1) Antibody in Flow Cytometry (Flow) — Staining of human erythroleukemia (HEL) cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD90 (Thy-1) PE (purple histogram). Total viable cells were used for analysis.

Product Details

12-0909-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.25 µg)/test	View 34 publications 34 publications

Product Specifications
Species Reactivity	Human
Published species	Horse, Human, Pig
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBio5E10 (5E10)
Conjugate	PE Alexa Fluor 700 APC APC-eFluor 780 Biotin FITC PE-Cyanine5 PE-Cyanine7 PerCP-Cyanine5.5 PerCP-eFluor 710 Super Bright 436 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10670624

Product Specific Information

Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometric analysis of human erythroleukemia (HEL) cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cells.

Inflammation and regeneration

Fujishiro A,Miura Y,Iwasa M,Fujii S,Sugino N,Andoh A,Hirai H,Maekawa T,Ichinohe T

12-0909 was used in Flow cytometry/Cell sorting to determine whether low dose irradiation affects the function of mesenchymal stromal/stem cells.

Fri Mar 11 00:00:00 EST 2022

Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.

Journal of extracellular vesicles

Zheng C,Sui B,Zhang X,Hu J,Chen J,Liu J,Wu D,Ye Q,Xiang L,Qiu X,Liu S,Deng Z,Zhou J,Liu S,Shi S,Jin Y

12-0909-42 was used in Flow Cytometry to investigate if functional efferocytosis of apoptotic vesicles restore liver macrophage homeostasis and ameliorates type 2 diabetes.

Sat May 01 00:00:00 EDT 2021

The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

International journal of molecular sciences

Gasiūnienė M,Valatkaitė E,Navakauskaitė A,Navakauskienė R

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Thu Nov 19 00:00:00 EST 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

Equine Dental Pulp Connective Tissue Particles Reduced Lameness in Horses in a Controlled Clinical Trial.

Frontiers in veterinary science

Bertone AL,Reisbig NA,Kilborne AH,Kaido M,Salmanzadeh N,Lovasz R,Sizemore JL,Scheuermann L,Kopp RJ,Zekas LJ,Brokken MT

12-0909 was used in Flow cytometry/Cell sorting to assess if injection of allogeneic dental pulp tissue particles improves lameness in horses with naturally occurring osteoarthritis or soft tissue injury.

Thu Oct 01 00:00:00 EDT 2020

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis.

Cell research

Wu J,Song D,Li Z,Guo B,Xiao Y,Liu W,Liang L,Feng C,Gao T,Chen Y,Li Y,Wang Z,Wen J,Yang S,Liu P,Wang L,Wang Y,Peng L,Stacey GN,Hu Z,Feng G,Li W,Huo Y,Jin R,Shyh-Chang N,Zhou Q,Wang L,Hu B,Dai H,Hao J

12-0909 was used in Flow cytometry/Cell sorting to investigate the use of hESC-derived immunity-regulatory and matrix-regulatory cells in treating lung injury and fibrosis in mice.

Tue Sep 01 00:00:00 EDT 2020

Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth.

Cells

Oh HA,Kwak J,Kim BJ,Jin HJ,Park WS,Choi SJ,Oh W,Um S

12-0909 was used in Flow cytometry/Cell sorting to suggest that P-CM can improve the efficacy in hair growth via a paracrine mechanism and that MIF in P-CM exerts hair growth-promoting effects via a VEGF-related β-catenin and p-GSK-3β [SER9] signaling pathway.

Thu May 28 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

BNC1 regulates cell heterogeneity in human pluripotent stem cell-derived epicardium.

Development (Cambridge, England)

Gambardella L,McManus SA,Moignard V,Sebukhan D,Delaune A,Andrews S,Bernard WG,Morrison MA,Riley PR,Göttgens B,Gambardella Le Novère N,Sinha S

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Fri Dec 13 00:00:00 EST 2019

Isolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties.

Cells

Rémy M,Ferraro F,Le Salver P,Rey S,Genot E,Djavaheri-Mergny M,Thébaud N,Boiziau C,Boeuf H

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Thu Nov 21 00:00:00 EST 2019

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.

Theranostics

Liu W,Zhang L,Xuan K,Hu C,Li L,Zhang Y,Jin F,Jin Y

12-0909 was used in Flow cytometry/Cell sorting to elucidate a novel role of tissue-nonspecific alkaline phosphatase in controlling bone marrow mesenchymal stem cell lineage selection.

Mon Sep 09 00:00:00 EDT 2019

Bone mesenchymal stromal cells exhibit functional inhibition but no chromosomal aberrations in chronic myelogenous leukemia.

Oncology letters

Xie J,Chen J,Wang B,He X,Huang H

12-0909-42 was used in Flow Cytometry to demonstrate functional inhibition of CML-BMSCs; however, no signs of chromosomal aberrations were observed, thereby providing insight into the changes occurring in the CML-BMM.

Tue Jan 01 00:00:00 EST 2019

Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress.

International journal of molecular sciences

Liu Y,Yang H,Wen Y,Li B,Zhao Y,Xing J,Zhang M,Chen Y

12-0909 was used in Flow cytometry/Cell sorting to analyze novel mechanisms underlying Nrf2-mediated anti-apoptosis in periodontal ligament stem cells in the periodontitis oxidative microenvironment.

Wed May 17 00:00:00 EDT 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.

Journal of translational medicine

Zakharova IS,Zhiven' MK,Saaya SB,Shevchenko AI,Smirnova AM,Strunov A,Karpenko AA,Pokushalov EA,Ivanova LN,Makarevich PI,Parfyonova YV,Aboian E,Zakian SM

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2017

Distinct signaling programs control human hematopoietic stem cell survival and proliferation.

Blood

Knapp DJ,Hammond CA,Aghaeepour N,Miller PH,Pellacani D,Beer PA,Sachs K,Qiao W,Wang W,Humphries RK,Sauvageau G,Zandstra PW,Bendall SC,Nolan GP,Hansen C,Eaves CJ

12-0909 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms by which distinct signalling programs control human hematopoietic stem cell survival and proliferation.

Thu Jan 19 00:00:00 EST 2017

Transendothelial migration of human umbilical mesenchymal stem cells across uterine endothelial monolayers: Junctional dynamics and putative mechanisms.

Placenta

Ebrahim NA,Leach L

12-0909 was used in Flow cytometry/Cell sorting to study the transmigration of foetal stem cells across maternal endothelial barriers, and investigate whether they can subsequently influence maternal endothelial integrity.

Thu Dec 01 00:00:00 EST 2016

Vitamin D machinery and metabolism in porcine adipose-derived mesenchymal stem cells.

Stem cell research & therapy

Valle YL,Almalki SG,Agrawal DK

12-0909 was used in Flow cytometry/Cell sorting to evaluate whether porcine adipose-derived MSCs can metabolise and respond to vitamin D.

Wed Aug 17 00:00:00 EDT 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Human cytomegalovirus infection of human adipose-derived stromal/stem cells restricts differentiation along the adipogenic lineage.

Adipocyte

Zwezdaryk KJ,Ferris MB,Strong AL,Morris CA,Bunnell BA,Dhurandhar NV,Gimble JM,Sullivan DE

12-0909 was used in Flow cytometry/Cell sorting to suggest that human cytomegalovirus (HCMV) infection during adipose-derived stromal/stem cell (ASC) therapy may have a poor outcome due to the virus interfering.

Wed May 04 00:00:00 EDT 2016

Satellite cell response to erythropoietin treatment and endurance training in healthy young men.

The Journal of physiology

Hoedt A,Christensen B,Nellemann B,Mikkelsen UR,Hansen M,Schjerling P,Farup J

12-0909 was used in Flow cytometry/Cell sorting to establish that endurance training and erythropoiesis-stimulating agent treatment may separately stimulate satellite cell commitment to the myogenic program.

Mon Feb 01 00:00:00 EST 2016

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2016

Impaired function of bone marrow stromal cells in systemic mastocytosis.

Stem cell research

Nemeth K,Wilson TM,Ren JJ,Sabatino M,Stroncek DM,Krepuska M,Bai Y,Robey PG,Metcalfe DD,Mezey E

12-0909 was used in Flow cytometry/Cell sorting to implicate epigenetic change in mesenchymal stem cells as a driver of some of the symptoms associated with systemic mastocytosis.

Wed Jul 01 00:00:00 EDT 2015

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.

International journal of clinical and experimental pathology

Yuan SM,Guo Y,Zhou XJ,Shen WM,Chen HN

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Tue May 26 00:00:00 EDT 2015

Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach.

Cytotechnology

Indumathi S,Harikrishnan R,Rajkumar JS,Dhanasekaran M

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 12-0909-42) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A