|Flow Cytometry (Flow)||5 µL (0.125 µg)/test|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.
Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometrric analysis of human erythroleukemia (HEL) cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD90; CDw90; Thy-1 antigen; Thy-1 membrane glycoprotein; Thy-1 T-cell antigen
Gene Aliases: CD90; CDw90; THY1
UniProt ID: (Human) P04216
Entrez Gene ID: (Human) 7070
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