Invitrogen

CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), PerCP-Cyanine5.5, eBioscience™

Product Image Gallery

CD90 (Thy-1) Antibody in Flow Cytometry (Flow) — Staining of the HEL cell line with Mouse IgG1 K Isotype Control PerCP-Cyanine5-5 (Product # 45-4714-82) (open histogram) or Anti-Human CD90 (Thy-1) PerCP-Cyanine5-5 (filled histogram). Total viable cells were used for analysis.

Product Details

45-0909-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.125 µg)/test	View 25 publications 25 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBio5E10 (5E10)
Conjugate	PerCP-Cyanine5.5 Alexa Fluor 700 APC APC-eFluor 780 Biotin FITC PE PE-Cyanine5 PE-Cyanine7 PerCP-eFluor 710 Super Bright 436 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10718245

Product Specific Information

Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometrric analysis of human erythroleukemia (HEL) cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

International journal of molecular sciences

Gasiūnienė M,Valatkaitė E,Navakauskaitė A,Navakauskienė R

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Thu Nov 19 00:00:00 EST 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

BNC1 regulates cell heterogeneity in human pluripotent stem cell-derived epicardium.

Development (Cambridge, England)

Gambardella L,McManus SA,Moignard V,Sebukhan D,Delaune A,Andrews S,Bernard WG,Morrison MA,Riley PR,Göttgens B,Gambardella Le Novère N,Sinha S

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Fri Dec 13 00:00:00 EST 2019

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Elderly human hematopoietic progenitor cells express cellular senescence markers and are more susceptible to pyroptosis.

JCI insight

Fali T,Fabre-Mersseman V,Yamamoto T,Bayard C,Papagno L,Fastenackels S,Zoorab R,Koup RA,Boddaert J,Sauce D,Appay V

45-0909 was used in Flow cytometry/Cell sorting to study blood haematopoietic progenitor cells from older humans.

Thu Jul 12 00:00:00 EDT 2018

Bone marrow niche trafficking of miR-126 controls the self-renewal of leukemia stem cells in chronic myelogenous leukemia.

Nature medicine

Zhang B,Nguyen LXT,Li L,Zhao D,Kumar B,Wu H,Lin A,Pellicano F,Hopcroft L,Su YL,Copland M,Holyoake TL,Kuo CJ,Bhatia R,Snyder DS,Ali H,Stein AS,Brewer C,Wang H,McDonald T,Swiderski P,Troadec E,Chen CC,Dorrance A,Pullarkat V,Yuan YC,Perrotti D,Carlesso N,Forman SJ,Kortylewski M,Kuo YH,Marcucci G

45-0909 was used in Flow cytometry/Cell sorting to demonstrate how bone marrow endothelial cells supply miR-126 to leukemia stem cells in chronic myelogenous leukemia which modulates their quiescence and self-renewal.

Tue May 01 00:00:00 EDT 2018

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

MALP-2, an agonist of TLR6, promotes the immune status without affecting the differentiation capacity of umbilical cord mesenchymal stem cells.

Experimental and therapeutic medicine

Wu X,Xu L,Shen Y,Yu N,Zhang Y,Guo T

45-0909 was used in Flow cytometry/Cell sorting to assess the role of TLR6 in the immune status of umbilical cord mesenchymal stem cells, by stimulation with the TLR6 agonist MALP-2.

Fri Dec 01 00:00:00 EST 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.

Journal of translational medicine

Zakharova IS,Zhiven' MK,Saaya SB,Shevchenko AI,Smirnova AM,Strunov A,Karpenko AA,Pokushalov EA,Ivanova LN,Makarevich PI,Parfyonova YV,Aboian E,Zakian SM

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2017

Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold.

PloS one

Sonomoto K,Yamaoka K,Kaneko H,Yamagata K,Sakata K,Zhang X,Kondo M,Zenke Y,Sabanai K,Nakayamada S,Sakai A,Tanaka Y

45-0909 was used in Flow cytometry/Cell sorting to investigate the combination of MSCs and NFs as a therapeutic technique for the repair of joints affected by bone and cartilage destruction.

Thu Sep 01 00:00:00 EDT 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2016

Adipose-derived Mesenchymal Stem Cells Are Phenotypically Superior for Regeneration in the Setting of Osteonecrosis of the Femoral Head.

Clinical orthopaedics and related research

Wyles CC,Houdek MT,Crespo-Diaz RJ,Norambuena GA,Stalboerger PG,Terzic A,Behfar A,Sierra RJ

45-0909 was used in Flow cytometry/Cell sorting to demonstrate that aMSCs outperform bmMSCs in growth rate and bone differentiation potential in osteonecrosis.

Thu Oct 01 00:00:00 EDT 2015

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.

International journal of clinical and experimental pathology

Yuan SM,Guo Y,Zhou XJ,Shen WM,Chen HN

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Tue May 26 00:00:00 EDT 2015

Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach.

Cytotechnology

Indumathi S,Harikrishnan R,Rajkumar JS,Dhanasekaran M

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Thu Dec 11 00:00:00 EST 2014

Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.

Tissue engineering. Part A

Mendez JJ,Ghaedi M,Steinbacher D,Niklason LE

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 45-0909-42) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2014

Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.

PloS one

Rosu-Myles M,She YM,Fair J,Muradia G,Mehic J,Menendez P,Prasad SS,Cyr TD

45-0909 was used in Flow cytometry/Cell sorting to provide evidence of heterogeneity among bone marrow stromal cells and identify potential candidate proteins for quantifying MSCs in vitro.