Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (cat. 11-0452) and 0.06 ug of Rat IgG2b kappa Isotype Control APC (cat. 17-4032) (left) or 0.06 ug of Anti-Mouse CD93 (AA4.1) APC (right). Total viable cells were used for analysis.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2b, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.125 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
Description: The AA4.1 monoclonal antibody reacts with mouse C1qRp, an approximately 130-140 kDa C-type lectin-like type I transmembrane protein. The AA4.1 antigen was originally identified as an antigen expressed on early stages of B cell development in bone marrow. Adult spleen and fetal liver also have detectable numbers of AA4.1^+ cells. In combination with other markers of hematopoietic progenitor cells such as Thy-1, Sca-1, c-Kit, CD43, and CD24, the bone marrow Lymphoid-Committed Progenitors (CLP) can be segregated into more primitive and more differentiated subsets based on expression of AA4.1. Correlated expression of surface IgM (sIgM), CD23, and AA4.1 antigen has also been used to define three nonproliferative subpopulations of immature/transitional peripheral B cells designated: T1 (AA4.1^+/CD23^-/sIgMhi), T2 (AA4.1^+/CD23^+/sIgMhi), and T3 (AA4.1^+/CD23^+/sIgMlo). AA4.1 is also reported to be expressed in cytoplasmic vesicles in endothelial cells, megakaryoblasts, and platelets. It is reported that monoclonal antibodies AA4.1 and 493 recognize different epitopes of the same molecule.
Applications Reported: The AA4.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The AA4.1 antibody has been tested by flow cytometric analysis of mouse bone marrow and splenocyte cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Molecular and cellular properties of the rat AA4 antigen, a C-type lectin-like receptor with structural homology to thrombomodulin.||Dean YD,McGreal EP,Akatsu H,Gasque P||The Journal of biological chemistry (275:34382)||2000|
|Not Applicable||Not Cited||Cell surface antigens expressed by subsets of pre-B cells and B cells.||McKearn JP,Baum C,Davie JM||Journal of immunology (Baltimore, Md. : 1950) (132:332)||1984|