Invitrogen

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Alexa Fluor 488, eBioscience™

Product Image Gallery

CD95 (APO-1/Fas) Antibody — Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82)

CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow) — Staining of BALB/c thymocytes with 1 µg of Mouse IgG1 kappa Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (blue histogram) or 1 µg of Anti-Mouse CD95 (APO-1/Fas) Alexa Fluor® 488 (purple histogram). Total viable cells were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

2 µg/test

Published Applications

Flow Cytometry (Flow)

See 7 publications below

Product Specifications

Species Reactivity

Mouse

Published species

Human

Host / Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

15A7

Conjugate

Alexa Fluor® 488

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_10671269

Product Specific Information

Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.

Applications Reported: This 15A7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ALPS1A; APO-1; Apo-1 antigen; Apo-1 Fas; Apoptosis-mediating surface antigen FAS; CD95; Fas (TNF receptor superfamily member); Fas antigen; FAS1; FASLG receptor; FASTM; lymphoproliferation; TNFRSF6; Tumor necrosis factor receptor superfamily member 6

Gene Aliases: AI196731; APO1; APT1; CD95; Fas; lpr; TNFR6; Tnfrsf6

UniProt ID: (Mouse) P25446

Entrez Gene ID: (Mouse) 14102

Molecular Function: cytokine receptor receptor tumor necrosis factor receptor