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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • FAS (CD95) Antibodies

          Invitrogen

          CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Alexa Fluor™ 488, eBioscience™

          Advanced Verification
          20 Published Figures
          13 References
          View all (118) FAS (CD95) antibodies

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          Cite CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Alexa Fluor™ 488, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (20)
          • Advanced Verification (1)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 22

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          CD95 (APO-1/Fas) Antibody (53-0951-82) in Flow

          BALB/c mouse thymocytes were stained with 1.0 µg of Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (blue histogram) or 1.0 µg of CD95 (APO-1/Fas) Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)
          CD95 (APO-1/Fas) Antibody

          Product Details

          53-0951-82

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          2 µg/test
          View 13 publications 13 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          15A7

          Conjugate

          Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488
          • Unconjugated
          • APC 
          • Biotin 
          • PE 
          • PerCP-eFluor 710
          • Super Bright 436
          • Request custom conjugation

          Excitation/Emission Max

          499/520 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10671269

          Product Specific Information

          Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.

          Applications Reported: This 15A7 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD95, also known as Fas or APO-1, is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor in the tumor necrosis factor receptor (TNFR) superfamily. It is expressed by a wide range of hematopoietic and non-hematopoietic cells, including monocytes, neutrophils, lymphocytes, and fibroblasts. CD95 also exists in a soluble form weighing approximately 26 kDa. The interaction of CD95 with its ligand, FasL, induces apoptosis, playing a crucial role in peripheral tolerance and anti-viral immune responses. Upon stimulation, CD95 leads to the aggregation of its intracellular Fas-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases. This cascade results in apoptosis. CD95 is also involved in activating NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and transduces proliferative signals in normal diploid fibroblasts and T cells. There are at least eight alternatively spliced transcript variants encoding seven distinct isoforms of CD95. Isoforms lacking the transmembrane domain may negatively regulate apoptosis mediated by the full-length isoform. The Fas/Fas ligand system is implicated in several human diseases, including AIDS, hepatitis, and cancer, highlighting its importance in immune regulation and disease pathology.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: Apo-1 antigen; Apo-1 Fas; Apo1 antigen; Apoptosis-mediating surface antigen FAS; CD95; Fas (TNF receptor superfamily member); Fas antigen; FASLG receptor; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; Tumor necrosis factor receptor superfamily member 6

          View more View less

          Gene Aliases: AI196731; APO1; APT1; CD95; Fas; lpr; TNFR6; Tnfrsf6

          View more View less

          UniProt ID: (Mouse) P25446

          View more View less

          Entrez Gene ID: (Mouse) 14102

          View more View less

          Function(s)
          protease binding transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding transmembrane signal receptor
          Process(es)
          immunoglobulin production renal system process apoptotic process activation-induced cell death of T cells inflammatory cell apoptotic process obsolete transformed cell apoptotic process immune response signal transduction extrinsic apoptotic signaling pathway via death domain receptors response to toxic substance gene expression regulation of gene expression positive regulation of cell death B cell mediated immunity dendrite regeneration positive regulation of protein homooligomerization tumor necrosis factor-mediated signaling pathway regulation of cell proliferation regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative thymic T cell selection regulation of lymphocyte differentiation regulation of myeloid cell differentiation spleen development negative regulation of B cell activation protein homooligomerization response to glucocorticoid neuron apoptotic process cellular response to lithium ion cellular response to hyperoxia positive regulation of release of cytochrome c from mitochondria motor neuron apoptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process necroptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.
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