Invitrogen

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Alexa Fluor™ 488, eBioscience™

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

19 Published Figures

12 References

View all (89) FAS (CD95) antibodies

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CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow) — BALB/c mouse thymocytes were stained with 1.0 µg of Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (blue histogram) or 1.0 µg of CD95 (APO-1/Fas) Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

CD95 (APO-1/Fas) Antibody — Figure 1 NKT Cell-Deficient Mice Exhibit Impaired Antiviral B Cell Responses (A and B) Flow cytometry analysis of mediastinal lymph nodes (LN) from control (black), wild-type (blue), or CD1d -/- (red) mice on day 9 of influenza infection. Contour plots display the percentage of (A) germinal center and (B) TfH cells. (C) Confocal microscopy images of mediastinal lymph node sections from mice treated as in (A) and (B), stained with antibodies to IgD (green) and GL7 (magenta). Scale bar, 450 mum. (D) Quantification of the number and follicular area occupied by germinal centers in confocal images shown in (C) by ImageJ. (E) ELISPOT analysis of IgG1 influenza-specific ASCs in mediastinal lymph nodes from wild-type and CD1d -/- mice treated as described in (A) and (B). (F and G) Flow cytometry analysis of mediastinal lymph nodes of from control (black), wild-type (blue), or CD1d -/- (red) mice on day 9 of vaccinia virus infection. Contour plots display the percentage of (F) germinal center and (G) TfH cells. Data show one representative result from three experiments, where each dot represents one mouse. Data represent mean +- SEM; two-tailed paired Student's t test, * p < 0.05, ** p < 0.01, and **** p < 0.0001. {TM}

Product Details

53-0951-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	2 µg/test	View 12 publications 12 publications

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	15A7
Conjugate	Alexa Fluor™ 488 APC Biotin PE PerCP-eFluor 710 Super Bright 436 Unconjugated
Excitation/Emission Max	499/520 nm View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10671269

Product Specific Information

Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.

Applications Reported: This 15A7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Elimination of receptor binding by influenza hemagglutinin improves vaccine-induced immunity.

NPJ vaccines

Hendin HE,Lavoie PO,Gravett JM,Pillet S,Saxena P,Landry N,D'Aoust MA,Ward BJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Mon Apr 11 00:00:00 EDT 2022

Induction of Foxp3 and activation of Tregs by HSP gp96 for treatment of autoimmune diseases.

iScience

Xu Y,Liu E,Xie X,Wang J,Zheng H,Ju Y,Chen L,Li C,Zhou X,Li Z,Li X,Meng S

53-0951-82 was used in Flow cytometry/Cell sorting to show that gp96 immunization restricted antibody-induced and Th-induced autoimmune diseases by integrating Treg expansion and activation, indicating its potential clinical usefulness against autoimmune diseases.

Fri Dec 17 00:00:00 EST 2021

Berberine modulates hyper-inflammation in mouse macrophages stimulated with polyinosinic-polycytidylic acid via calcium-CHOP/STAT pathway.

Scientific reports

Kim HJ,Kim YJ,Park W

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Fri May 28 00:00:00 EDT 2021

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

γδ T cells control humoral immune response by inducing T follicular helper cell differentiation.

Nature communications

Rezende RM,Lanser AJ,Rubino S,Kuhn C,Skillin N,Moreira TG,Liu S,Gabriely G,David BA,Menezes GB,Weiner HL

53-0951 was used in Flow cytometry/Cell sorting to demonstrate that complete Freund's adjuvant immunisation induces a subpopulation of CXCR5-expressing γδ T cells in the draining lymph nodes.

Wed Aug 08 00:00:00 EDT 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment.

Scientific reports

Yeku OO,Purdon TJ,Koneru M,Spriggs D,Brentjens RJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Tue Sep 05 00:00:00 EDT 2017

YY1 plays an essential role at all stages of B-cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Kleiman E,Jia H,Loguercio S,Su AI,Feeney AJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Tue Jul 05 00:00:00 EDT 2016

The histone lysine methyltransferase KMT2D sustains a gene expression program that represses B cell lymphoma development.

Nature medicine

Ortega-Molina A,Boss IW,Canela A,Pan H,Jiang Y,Zhao C,Jiang M,Hu D,Agirre X,Niesvizky I,Lee JE,Chen HT,Ennishi D,Scott DW,Mottok A,Hother C,Liu S,Cao XJ,Tam W,Shaknovich R,Garcia BA,Gascoyne RD,Ge K,Shilatifard A,Elemento O,Nussenzweig A,Melnick AM,Wendel HG

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age.

The Journal of biological chemistry

Zhao H,Bauzon F,Bi E,Yu JJ,Fu H,Lu Z,Cui J,Jeon H,Zang X,Ye BH,Zhu L

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Fri Feb 27 00:00:00 EST 2015

Up-regulation of Fas (CD95) expression in tumour cells in vivo.

Immunology

Peshes-Yaloz N,Rosen D,Sondel PM,Krammer PH,Berke G

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Apo-1 antigen; Apo-1 Fas; Apo1 antigen; Apoptosis-mediating surface antigen FAS; CD95; Fas (TNF receptor superfamily member); Fas antigen; FASLG receptor; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; Tumor necrosis factor receptor superfamily member 6

Gene Aliases: AI196731; APO1; APT1; CD95; Fas; lpr; TNFR6; Tnfrsf6

UniProt ID: (Mouse) P25446

Entrez Gene ID: (Mouse) 14102

Molecular Function: transmembrane signal receptor

Function(s)

immunoglobulin production renal system process apoptotic process activation-induced cell death of T cells inflammatory cell apoptotic process obsolete transformed cell apoptotic process immune response signal transduction extrinsic apoptotic signaling pathway via death domain receptors response to toxic substance gene expression regulation of gene expression positive regulation of cell death B cell mediated immunity dendrite regeneration positive regulation of protein homooligomerization tumor necrosis factor-mediated signaling pathway regulation of cell proliferation regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative thymic T cell selection regulation of lymphocyte differentiation regulation of myeloid cell differentiation spleen development negative regulation of B cell activation protein homooligomerization response to glucocorticoid neuron apoptotic process cellular response to lithium ion cellular response to hyperoxia positive regulation of release of cytochrome c from mitochondria motor neuron apoptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process necroptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway in absence of ligand

Process(es)

protease binding transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Alexa Fluor™ 488, eBioscience™

Product Details

53-0951-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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