Invitrogen

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Alexa Fluor™ 488, eBioscience™

CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)

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CD95 (APO-1/Fas) Antibody (53-0951-82) in Flow

BALB/c mouse thymocytes were stained with 1.0 µg of Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (blue histogram) or 1.0 µg of CD95 (APO-1/Fas) Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

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Product Details

53-0951-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

2 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

15A7

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10671269

Product Specific Information

Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.

Applications Reported: This 15A7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD95, also known as Fas or APO-1, is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor in the tumor necrosis factor receptor (TNFR) superfamily. It is expressed by a wide range of hematopoietic and non-hematopoietic cells, including monocytes, neutrophils, lymphocytes, and fibroblasts. CD95 also exists in a soluble form weighing approximately 26 kDa. The interaction of CD95 with its ligand, FasL, induces apoptosis, playing a crucial role in peripheral tolerance and anti-viral immune responses. Upon stimulation, CD95 leads to the aggregation of its intracellular Fas-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases. This cascade results in apoptosis. CD95 is also involved in activating NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and transduces proliferative signals in normal diploid fibroblasts and T cells. There are at least eight alternatively spliced transcript variants encoding seven distinct isoforms of CD95. Isoforms lacking the transmembrane domain may negatively regulate apoptosis mediated by the full-length isoform. The Fas/Fas ligand system is implicated in several human diseases, including AIDS, hepatitis, and cancer, highlighting its importance in immune regulation and disease pathology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2022

Elimination of receptor binding by influenza hemagglutinin improves vaccine-induced immunity.

NPJ vaccines

Hendin HE,Lavoie PO,Gravett JM,Pillet S,Saxena P,Landry N,D'Aoust MA,Ward BJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Mon Apr 11 00:00:00 UTC 2022

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Thu Apr 01 00:00:00 UTC 2021

Berberine modulates hyper-inflammation in mouse macrophages stimulated with polyinosinic-polycytidylic acid via calcium-CHOP/STAT pathway.

Scientific reports

Kim HJ,Kim YJ,Park W

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Fri May 28 00:00:00 UTC 2021

Induction of Foxp3 and activation of Tregs by HSP gp96 for treatment of autoimmune diseases.

iScience

Xu Y,Liu E,Xie X,Wang J,Zheng H,Ju Y,Chen L,Li C,Zhou X,Li Z,Li X,Meng S

53-0951-82 was used in Flow cytometry/Cell sorting to show that gp96 immunization restricted antibody-induced and Th-induced autoimmune diseases by integrating Treg expansion and activation, indicating its potential clinical usefulness against autoimmune diseases.

Fri Dec 17 00:00:00 UTC 2021

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Thu Jan 25 00:00:00 UTC 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Mon Mar 19 00:00:00 UTC 2018

γδ T cells control humoral immune response by inducing T follicular helper cell differentiation.

Nature communications

Rezende RM,Lanser AJ,Rubino S,Kuhn C,Skillin N,Moreira TG,Liu S,Gabriely G,David BA,Menezes GB,Weiner HL

53-0951 was used in Flow cytometry/Cell sorting to demonstrate that complete Freund's adjuvant immunisation induces a subpopulation of CXCR5-expressing γδ T cells in the draining lymph nodes.

Wed Aug 08 00:00:00 UTC 2018

Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment.

Scientific reports

Yeku OO,Purdon TJ,Koneru M,Spriggs D,Brentjens RJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Tue Sep 05 00:00:00 UTC 2017

YY1 plays an essential role at all stages of B-cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Kleiman E,Jia H,Loguercio S,Su AI,Feeney AJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Tue Jul 05 00:00:00 UTC 2016

The histone lysine methyltransferase KMT2D sustains a gene expression program that represses B cell lymphoma development.

Nature medicine

Ortega-Molina A,Boss IW,Canela A,Pan H,Jiang Y,Zhao C,Jiang M,Hu D,Agirre X,Niesvizky I,Lee JE,Chen HT,Ennishi D,Scott DW,Mottok A,Hother C,Liu S,Cao XJ,Tam W,Shaknovich R,Garcia BA,Gascoyne RD,Ge K,Shilatifard A,Elemento O,Nussenzweig A,Melnick AM,Wendel HG

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Thu Oct 01 00:00:00 UTC 2015

Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age.

The Journal of biological chemistry

Zhao H,Bauzon F,Bi E,Yu JJ,Fu H,Lu Z,Cui J,Jeon H,Zang X,Ye BH,Zhu L

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Fri Feb 27 00:00:00 UTC 2015

Up-regulation of Fas (CD95) expression in tumour cells in vivo.

Immunology

Peshes-Yaloz N,Rosen D,Sondel PM,Krammer PH,Berke G

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 53-0951-82) in Flow Cytometry

Sun Apr 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: Apo-1 antigen; Apo-1 Fas; Apo1 antigen; Apoptosis-mediating surface antigen FAS; CD95; Fas (TNF receptor superfamily member); Fas antigen; FASLG receptor; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; Tumor necrosis factor receptor superfamily member 6

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Gene Aliases: AI196731; APO1; APT1; CD95; Fas; lpr; TNFR6; Tnfrsf6

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UniProt ID: (Mouse) P25446

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Entrez Gene ID: (Mouse) 14102

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Function(s)

protease binding transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding transmembrane signal receptor

Process(es)

immunoglobulin production renal system process apoptotic process activation-induced cell death of T cells inflammatory cell apoptotic process obsolete transformed cell apoptotic process immune response signal transduction extrinsic apoptotic signaling pathway via death domain receptors response to toxic substance gene expression regulation of gene expression positive regulation of cell death B cell mediated immunity dendrite regeneration positive regulation of protein homooligomerization tumor necrosis factor-mediated signaling pathway regulation of cell proliferation regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative thymic T cell selection regulation of lymphocyte differentiation regulation of myeloid cell differentiation spleen development negative regulation of B cell activation protein homooligomerization response to glucocorticoid neuron apoptotic process cellular response to lithium ion cellular response to hyperoxia positive regulation of release of cytochrome c from mitochondria motor neuron apoptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process necroptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway in absence of ligand

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CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Alexa Fluor™ 488, eBioscience™

CD95 (APO-1/Fas) Antibody (53-0951-82) in Flow

Product Details

53-0951-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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