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Immunofluorescent analysis of CD99 (green) showing staining in the membrane of Jurkat cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD99 monoclonal antibody (Product # MA5-12954) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Purified E-rosette forming cells from human peripheral blood lymphocytes|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-200|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 4 publications below|
MA5-12954 targets CD99 in WB, IF, FACS and IHC (P) applications and shows reactivity with Human and Rat samples. This antibody is not suitable for Mouse spleen cells in Western blot analysis.
The MA5-12954 immunogen is purified E-rosette forming cells from human peripheral blood lymphocytes.
CD99, or MIC2 gene product, or E2 antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. The antigen is also expressed by most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Pericardial synovial sarcoma, a potential for misdiagnosis: clinicopathologic and molecular cytogenetic analysis of three cases with literature review.
MA5-12954 was used in immunohistochemistry to report on three cases of pericardial synovial sarcoma
|Cheng Y,Sheng W,Zhou X,Wang J||American journal of clinical pathology (137:142)||2012|
Extraventricular neurocytomas: a morphological and histogenetic consideration. A study of six cases.
MA5-12954 was used in immunohistochemistry to study the morphological and histological features of neurocytomas in extraventricular locations
|Agarwal S,Sharma MC,Sarkar C,Suri V,Jain A,Sharma MS,Ailawadhi P,Garg A,Mallick S||Pathology (43:327)||2011|
Primary sellar neuroblastoma presenting with syndrome of inappropriate secretion of anti-diuretic hormone.
MA5-12954 was used in immunohistochemistry to investigate the secretion of anti-diuretic hormone in neuroblastoma
|Radotra B,Apostolopoulos V,Sandison A,Hatfield EC,Mendoza N,Moss J,Mehta A,Glaser M,Meeran K,Roncaroli F||Endocrine pathology (21:266)||2010|
Uterine carcinosarcoma metastatic to the lung as large-cell neuroendocrine carcinoma with synchronous sarcoid granulomatosis.
MA5-12954 was used in immunohistochemistry to report a clinical case of uterine carcinosarcoma with unique features
|Froio E,D'Adda T,Fellegara G,Martella E,Caruana P,Pruneri G,Pesci A,Rindi G||Lung cancer (Amsterdam, Netherlands) (64:371)||2009|
antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99 antigen; cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; E2 antigen; HBA71; MIC2; MIC2 (monoclonal antibody 12E7); MIC2X; MIC2Y; MSK5X; surface antigen MIC2; T-cell surface glycoprotein E2
CD99; HBA71; MIC2; MIC2X; MIC2Y; MSK5X