Immunofluorescence analysis of CD99 was performed using 90% confluent log phase MOLT-4 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD99 / MIC2 (O13) Mouse Monoclonal Antibody (MA5-12287) at 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in the membrane. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MeWo human melanoma cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12287 targets CD99 in IHC (P) applications and shows reactivity with Human samples.
The MA5-12287 immunogen is meWo human melanoma cells.
CD99, or MIC2 gene product, or E2 antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. The antigen is also expressed by most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Histopathological, immunophenotypic and clinical particularities and evolution of a case of hepatosplenic T-cell lymphoma in transformation to leukemia.
MA5-12287 was used in immunohistochemistry to report on a case of hepatosplenic T-cell lymphoma with leukemic transformation
|Benedek Lázár E,Köpeczi JB,Tunyogi AB,Kakucs E,Horváth E,Turcu M,Benedek I||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (54:1135)||2014|
Kit expression in spindle cell rhabdomyosarcoma can possibly create a different approach for its tumorigenesis and therapy.
MA5-12287 was used in immunohistochemistry to investigate the tumorigenesis of rhabdomyosarcoma through Kit expression and its therapeutic implication
|Diniz G,Aktas S,Ortac R,Tunakan M,Unlu I,Vergin C||Pathology, research and practice (202:671)||2006|
MiR-30a-5p connects EWS-FLI1 and CD99, two major therapeutic targets in Ewing tumor.
MA5-12287 was used in flow cytometry to study the interaction of miRNA-30a-5p with the Ewing sarcoma therapeutic targets EWS-FLI1 and CD99
|Franzetti GA,Laud-Duval K,Bellanger D,Stern MH,Sastre-Garau X,Delattre O||Oncogene (32:3915)||2013|
Inducible expression of chimeric EWS/ETS proteins confers Ewing's family tumor-like phenotypes to human mesenchymal progenitor cells.
MA5-12287 was used in immunocytochemistry to study the conferment of a Ewing's family tumor phenotype on human bone marrow-derrived mesenchymal progenitor cells by ectopic expression of EWS-ETS fusion proteins
|Miyagawa Y,Okita H,Nakaijima H,Horiuchi Y,Sato B,Taguchi T,Toyoda M,Katagiri YU,Fujimoto J,Hata J,Umezawa A,Kiyokawa N||Molecular and cellular biology (28:2125)||2008|