Invitrogen

CDC123 Monoclonal Antibody (OTI1F8)

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CDC123 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CDC123 in COS7 cells. Cells were transfected with a plasmid overexpressing CDC123 and probed with a CDC123 monoclonal antibody (Product # MA5-26184).

CDC123 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CDC123 monoclonal antibody (Product # MA5-26184).

CDC123 Antibody in Western Blot (WB) — Western blot analysis of CDC123 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with CDC123 (Product # MA5-26184) monoclonal antibody.

Product Details

MA5-26184

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:4000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Dog, Human, Non-human primate
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI1F8
Immunogen	Full length human recombinant protein of CDC123 produced in HEK293T cell View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2722977

Target Information

The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid. The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase, and a homodimeric dihydrolipoamide dehydrogenase. E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.