Invitrogen

CDC123 Monoclonal Antibody (OTI1F8)

Product Image Gallery

CDC123 Antibody in Western Blot (WB) — Western blot analysis of CDC123 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with CDC123 (Product # MA5-26184) monoclonal antibody.

CDC123 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human kidney tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a CDC123 monoclonal antibody (Product # MA5-26184).

CDC123 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CDC123 in COS7 cells. Cells were transfected with a plasmid overexpressing CDC123 and probed with a CDC123 monoclonal antibody (Product # MA5-26184).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:4000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Dog, Human, Non-human primate

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI1F8

Immunogen

Full length human recombinant protein of CDC123 produced in HEK293T cell

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2722977

Target Information

The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid. The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase, and a homodimeric dihydrolipoamide dehydrogenase. E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.