Immunohistochemistry analysis of CDC2/CDK1 showing staining in the cytoplasm and membrane of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CDC2/CDK1 mouse monoclonal Antibody (331800) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Amphibian, Chicken, Human, Mouse, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant protein consisiting of the carboxy-terminal two-thirds of the Xenopus p34cdc2|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||5-10 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Immunoprecipitation (IP)||5 ug|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor , which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Enhanced expression of mitochondrial superoxide dismutase leads to prolonged in vivo cell cycle progression and up-regulation of mitochondrial thioredoxin.
33-1800 was used in immunohistochemistry - paraffin section to investigate the role of Mn superoxide dismutase in tissue regeneration using transgenic mice
|Kim A,Joseph S,Khan A,Epstein CJ,Sobel R,Huang TT||Free radical biology and medicine (48:1501)||2010|
|Human||Not Cited||CDC2/CDK1 expression in esophageal adenocarcinoma and precursor lesions serves as a diagnostic and cancer progression marker and potential novel drug target.||Hansel DE,Dhara S,Huang RC,Ashfaq R,Deasel M,Shimada Y,Bernstein HS,Harmon J,Brock M,Forastiere A,Washington MK,Maitra A,Montgomery E||The American journal of surgical pathology (29:390)||2005|
|Human||Not Cited||Alpha(v)beta3 integrin expression up-regulates cdc2, which modulates cell migration.||Manes T,Zheng DQ,Tognin S,Woodard AS,Marchisio PC,Languino LR||The Journal of cell biology (161:817)||2003|