Immunohistochemistry analysis of CDC25C (25C14 (TC-14)) showing staining in the nucleus and weak cytoplasm of paraffin-embedded human testis tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CDC25C Antibody (25C14 (TC-14)) Mouse Monoclonal Antibody (MA514107) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human CDC25C protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1-3 ul|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-14107 targets CDC25C in IF, IP, ChIP and WB applications and shows reactivity with Human samples.
The MA5-14107 immunogen is recombinant human CDC25C protein.
Cyclin-dependent kinases can be activated by CDC25, which removes inhibitory phosphates from tyrosine and threonine residues. At least three CDC25 genes (CDC25A, CDC25B, and CDC25C) have been identified in humans. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HYAL1 hyaluronidase in prostate cancer: a tumor promoter and suppressor.
MA5-14107 was used in western blot to investigate the role of the HYAL1 hyaluronidase in the pathogenesis of prostate cancer
|Lokeshwar VB,Cerwinka WH,Isoyama T,Lokeshwar BL||Cancer research (65:7782)||2005|