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|Tested species reactivity||Human|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human CDC6 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
MA5-14314 targets CDC6 in IP and WB applications and shows reactivity with Human samples.
The MA5-14314 immunogen is recombinant human CDC6 protein.
The replication licensing system acts to ensure that no section of the genome is replicated more than once in a single cell cycle. The origin recognition complex (ORC) and CDC6/CDC18 are needed on chromatin before the licensing reaction can take place. During the transition from a growth-arrested to a proliferative state, transcription of mammalian CDC6 is regulated by the cell cycle transcription factor (E2F protein). Immunoblots show that minichromosome maintenance (MCM) proteins are not associated with chromatin. Competence of G1 phase nuclei to replicate in vitro coincides with maximum CDC6 accumulation and MCM protein binding to chromatin in vivo. Antibodies against CDC6 and MCM5 stain abnormal (neoplastically transformed) cells in cervical smears and sections with remarkably high specificity and sensitivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27 protein undergoes C-terminal phosphorylation.
MA5-14314 was used in western blot to study the mechanism of Cdk2 activation by Cdc6 protein
|Uranbileg B,Yamamoto H,Park JH,Mohanty AR,Arakawa-Takeuchi S,Jinno S,Okayama H||The Journal of biological chemistry (287:6275)||2012|
Inactivation and disassembly of the anaphase-promoting complex during human cytomegalovirus infection is associated with degradation of the APC5 and APC4 subunits and does not require UL97-mediated phosphorylation of Cdh1.
MA5-14314 was used in western blot to study the molecular mechanism underlying anaphase-promoting complex inactivation during human CMV infection
|Tran K,Kamil JP,Coen DM,Spector DH||Journal of virology (84:10832)||2010|
First Cdc7 kinase inhibitors: pyrrolopyridinones as potent and orally active antitumor agents. 2. Lead discovery.
MA5-14314 was used in western blot to evaluate the antitumor effect of various cdc7 kinase inhibitors
|Menichincheri M,Bargiotti A,Berthelsen J,Bertrand JA,Bossi R,Ciavolella A,Cirla A,Cristiani C,Croci V,D'Alessio R,Fasolini M,Fiorentini F,Forte B,Isacchi A,Martina K,Molinari A,Montagnoli A,Orsini P,Orzi F,Pesenti E,Pezzetta D,Pillan A,Poggesi I,Roletto F,Scolaro A,Tatò M,Tibolla M,Valsasina B,Varasi M,Volpi D,Santocanale C,Vanotti E||Journal of medicinal chemistry (52:293)||2009|
RNA-dependent recruitment of the origin recognition complex.
MA5-14314 was used in western blot to examine the mechanism for the recruitment of origin recognition complex
|Norseen J,Thomae A,Sridharan V,Aiyar A,Schepers A,Lieberman PM||The EMBO journal (27:3024)||2008|
CDC18 (cell division cycle 18; cdc18-related protein; CDC6 cell division cycle 6 homolog; CDC6-related protein; cell division control protein 6 homolog; cell division cycle 6 homolog; cell division cycle 6 protein; homolog)-like; p62(cdc6); S.pombe
CDC18L; CDC6; HsCDC18; HsCDC6