|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GST fusion protein full length CDC7|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12589 targets CDC7 Kinase in IHC (P), IP, and WB applications and shows reactivity with Human samples.
The MA5-12589 immunogen is gST fusion protein full length CDC7.
CDC7, a catalytic subunit of Dbf4 is an important regulator of S-phase in human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Increased Cdc7 expression is a marker of oral squamous cell carcinoma and overexpression of Cdc7 contributes to the resistance to DNA-damaging agents.
MA5-12589 was used in immunohistochemistry and western blot to study the utility of Csc7 as a marker of squamous cell carcinoma and its role in resistance to genotoxic therapeutic agents
|Cheng AN,Jiang SS,Fan CC,Lo YK,Kuo CY,Chen CH,Liu YL,Lee CC,Chen WS,Huang TS,Wang TY,Lee AY||Cancer letters (337:218)||2013|
Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity.
MA5-12589 was used in western blot to study the interaction between lens epithelium-derived growth factor and Cdc7-activator of S-phase kinase
|Hughes S,Jenkins V,Dar MJ,Engelman A,Cherepanov P||The Journal of biological chemistry (285:541)||2010|
Inhibition of Cdc7/Dbf4 kinase activity affects specific phosphorylation sites on MCM2 in cancer cells.
MA5-12589 was used in western blot to study the ability of Cdc7/Dbf4 kinase to phosphorylate two novel sites on MCM2 in cancer cells
|Charych DH,Coyne M,Yabannavar A,Narberes J,Chow S,Wallroth M,Shafer C,Walter AO||Journal of cellular biochemistry (104:1075)||2008|
Identification of Mcm2 phosphorylation sites by S-phase-regulating kinases.
MA5-12589 was used in western blot to investigate Mcm2 phosphorylation sites by different kinases involved in regulation of S phase progression
|Montagnoli A,Valsasina B,Brotherton D,Troiani S,Rainoldi S,Tenca P,Molinari A,Santocanale C||The Journal of biological chemistry (281:10281)||2006|
Geminin is bound to chromatin in G2/M phase to promote proper cytokinesis.
MA5-12589 was used in western blot to study the importance of G2/M phase geminin-chromatin binding for correct cytokinesis
|Nakuci E,Xu M,Pujana MA,Valls J,Elshamy WM||The international journal of biochemistry and cell biology (38:1207)||2006|
|Not Applicable||Not Cited||
Geminin overexpression prevents the completion of topoisomerase II¿ chromosome decatenation, leading to aneuploidy in human mammary epithelial cells.
MA5-12589 was used in immunoprecipitation and western blot to study the role of geminin in topoisomerase II chromosome decatenation and aneuploidy in human mammary epithelial cells
|Gardner L,Malik R,Shimizu Y,Mullins N,ElShamy WM||Breast cancer research : BCR (13:null)||2011|