Western blot analysis of CDCA7L/RAM2 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using a CDCA7L/RAM2 polyclonal antibody (Product # PA5-23443) at 4 ug/ml and 2 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody.
|Tested species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (186 CRQVIQREDSTSESEDDSR 204) of CDCA7L/RAM2 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Cell division cycle-associated 7-like protein (or RAM2) is a transcription factor which plays a role in transcriptional regulation and apoptotic signaling pathways. It acts as a repressor that inhibits monoamine oxidase A (MAOA) activity and gene expression by binding to promoter. It also plays an important oncogenic role in mediating the full transforming effect of MYC in medulloblastoma cells. It may act downstream of P38-kinase and BCL-2, but upstream of CASP3/caspase-3 as well as CCND1/cyclin D1 and E2F1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.